Cell Culture Engineering

  • Editors
  • Wei-Shou Hu

Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 101)

Table of contents

  1. Front Matter
    Pages I-XII
  2. Katie F. Wlaschin, Wei-Shou Hu
    Pages 43-74
  3. Konstantin Konstantinov, Chetan Goudar, Maria Ng, Renato Meneses, John Thrift, Sandy Chuppa et al.
    Pages 75-98
  4. Chetan Goudar, Richard Biener, Chun Zhang, James Michaels, James Piret, Konstantin Konstantinov
    Pages 99-118
  5. Gargi Seth, Patrick Hossler, Joon Chong Yee, Wei-Shou Hu
    Pages 119-164
  6. Back Matter
    Pages 165-168

About this book


Since the introduction of recombinant human growth hormone and insulin a quarter century ago, protein therapeutics has greatly broadened the ho- zon of health care. Many patients suffering with life-threatening diseases or chronic dysfunctions, which were medically untreatable not long ago, can attest to the wonder these drugs have achieved. Although the ?rst generation of p- tein therapeutics was produced in recombinant Escherichia coli, most recent products use mammalian cells as production hosts. Not long after the ?rst p- duction of recombinant proteins in E. coli, it was realized that the complex tasks of most post-translational modi?cations on proteins could only be ef?ciently carried out in mammalian cells. In the 1990s, we witnessed a rapid expansion of mammalian-cell-derived protein therapeutics, chie?y antibodies. In fact, it has been nearly a decade since the market value of mammalian-cell-derived protein therapeutics surpassed that of those produced from E. coli. A common characteristic of recent antibody products is the relatively large dose required for effective therapy, demanding larger quantities for the treatment of a given disease. This, coupled with the broadening repertoire of protein drugs, has rapidly expanded the quantity needed for clinical applications. The increasing demand for protein therapeutics has not been met exclusively by construction of new manufacturing plants and increasing total volume capacity. More - portantly the productivity of cell culture processes has been driven upward by an order of magnitude in the past decade.


Biotechnology Cell Culture Escherichia coli biochemical engineering cell biology post-translational modification protein proteins translation

Bibliographic information