Handbook of Single-Molecule Biophysics

  • Peter Hinterdorfer
  • Antoine Oijen

Table of contents

  1. Front Matter
    Pages i-xxi
  2. Thomas Schmidt, Gerhard J. Schütz
    Pages 19-42
  3. Ling Chin Hwang, Johannes Hohlbein, Seamus J. Holden, Achillefs N. Kapanidis
    Pages 129-163
  4. Joseph J. Loparo, Antoine van Oijen
    Pages 165-182
  5. Teuta Pilizota, Yoshiyuki Sowa, Richard M. Berry
    Pages 183-216
  6. Thomas Weidemann, Petra Schwille
    Pages 217-241
  7. Meni Wanunu, Gautam V. Soni, Amit Meller
    Pages 265-291
  8. Michael T. Woodside, Megan T. Valentine
    Pages 341-370
  9. I. D. Vilfan, J. Lipfert, D. A. Koster, S. G. Lemay, N. H. Dekker
    Pages 371-395
  10. Michael Schlierf, Matthias Rief
    Pages 397-406
  11. Andreas Ebner, Reinat Nevo, Christian Ranki, Johannes Preiner, Hermann Gruber, Ruti Kapon et al.
    Pages 407-447
  12. S. Scheuring, K. Tanuj Sapra, Daniel J. Müller
    Pages 449-485
  13. Toshio Ando, Takayuki Uchihashi
    Pages 487-523
  14. Andreas Ebner, Lilia Chtcheglova, Jilin Tang, David Alsteens, Vincent Dupres, Yves F. Dufrêne et al.
    Pages 525-554
  15. Xiaohui Zhang, Felix Rico, Amy J. Xu, Vincent T. Moy
    Pages 555-570
  16. Evan Evans, Ken Halvorsen, Koji Kinoshita, Wesley P. Wong
    Pages 571-589
  17. Cheng Zhu, Veronika I. Zarnitsyna, Wei Chen, Krishna Sarangapani
    Pages 591-610
  18. Back Matter
    Pages 611-626

About this book


The last decade has seen the development of a number of novel biophysical methods that allow the manipulation and study of individual biomolecules. The ability to monitor biological processes at this fundamental level of sensitivity has given rise to an improved understanding of the underlying molecular mechanisms. Through the removal of ensemble averaging, distributions and fluctuations of molecular properties can be characterized, transient intermediates identified, and catalytic mechanisms elucidated. By applying forces on biomolecules while monitoring their activity, important information can be obtained on how proteins couple function to structure. The Handbook of Single-Molecule Biophysics provides an introduction to these techniques and presents an extensive discussion of the new biological insights obtained from them. Coverage includes:

  • Experimental techniques to monitor and manipulate individual biomolecules
  • The use of single-molecule techniques in super-resolution and functional imaging
  • Single-molecule studies of physical properties of biomolecules
  • Single-molecule enzymology and biochemistry
  • Single molecules in the membrane
  • Single-molecule techniques in living cells
  • Integration of single-molecule biophysics and nanoscience


Biomembran Biophysics FRET cells fluorescence imaging microscopy molecule proteins

Editors and affiliations

  • Peter Hinterdorfer
    • 1
  • Antoine Oijen
    • 2
  1. 1.Institut für BiophysikUniversität LinzLinzAustria
  2. 2.Harvard Medical SchoolHarvard UniversityBostonU.S.A.

Bibliographic information