Methods in Protein Sequence Analysis

  • Marshall Elzinga

Part of the Experimental Biology and Medicine book series (EBAM, volume 3)

Table of contents

  1. Front Matter
    Pages i-xxvii
  2. Conference Lecture

    1. Front Matter
      Pages xxvii-xxvii
  3. Overview

    1. Front Matter
      Pages 25-25
    2. Brigitte Wittmann-Liebold
      Pages 27-63
  4. Strategy

    1. Front Matter
      Pages 65-65
    2. Kenneth A. Walsh
      Pages 67-73
  5. Improvements in Instrumentation and the Chemistry of Isothiocyanate Degradation

    1. Front Matter
      Pages 75-75
    2. Michael W. Hunkapiller, Rodney M. Hewick, William J. Dreyer, Leroy E. Hood
      Pages 77-90
  6. Microsequencing

    1. Front Matter
      Pages 111-111
    2. J. Y. Chang, R. Knecht, D. G. Braun
      Pages 113-118
    3. Ajit S. Bhown, John E. Mole, J. Claude Bennett
      Pages 119-130
    4. Joachim Spiess, Jean Rivier, Catherine Rivier, Wylie Vale
      Pages 131-138
    5. Konrad Beyreuther, Barbara Bieseler, Ruth Ehring, Benno Müller-Hill
      Pages 139-148
    6. Lawrence K. Duffy, Alexander Kurosky
      Pages 149-156
  7. Solid Phase Sequencing

    1. Front Matter
      Pages 157-157
    2. Johann Salnikow, Arnold Lehmann, Brigitte Wittmann-Liebold
      Pages 181-188
    3. Michael N. Margolies, Andrew Brauer, Christine Oman, David G. Klapper, Marcus J. Horn
      Pages 189-203
    4. Stephen B. H. Kent, Mark Riemen, Marie LeDoux, R. B. Merrifield
      Pages 205-213
    5. Alex G. Bonner, Marcus J. Horn, Richard S. Neves, Stephen B. H. Kent
      Pages 215-219
  8. Manual Sequencing

    1. Front Matter
      Pages 221-221
    2. George E. Tarr
      Pages 223-232
    3. Clyde Zalut
      Pages 233-239
  9. Sequencing by Mass Spectrometry

    1. Front Matter
      Pages 241-241
    2. H. R. Morris, G. W. Taylor, M. Panico, A. Dell, A. T. Etienne, R. A. McDowell et al.
      Pages 243-261
  10. Cleavage of Proteins

    1. Front Matter
      Pages 289-289
    2. B. Keil
      Pages 291-304
    3. Mark A. Hermodson
      Pages 313-323
    4. A. Fontana, D. Dalzoppo, C. Grandi, M. Zambonin
      Pages 325-334
  11. Coordination of Protein and DNA Sequencing

    1. Front Matter
      Pages 335-335
    2. John E. Walker, Alex Eberle, Nicholas J. Gay, Peter Hanisch, Matti Saraste, Michael J. Runswick
      Pages 337-354
  12. Use of High Performance Liquid Chromatrography (HPLC) for the Separation of Proteins, Peptides, and Amino Acids

About this book


Methods in Protein Sequence Analysis contains an intensely prac­ tical account of all the new methodology available to scientists carrying out protein and peptide sequencing studies. Many of the striking advances in fields as diverse as immunology, cell motility, and neurochemistry have in fact been fueled by our ever more powerful ability to determine the sequences and structures of key proteins and peptides. It is our hope that the rich array of tech­ niques and methods for sequencing proteins discussed in this volume-methods that generate much of the information crucial to progress in modern biology-will now become accessible to all who can benefit from them. The papers of the present volume constitute the Proceedings of the IVth International Conference on Methods in Protein Se­ quence Analysis, which was held at Brookhaven National Labo­ ratory, Upton, NY, September 21-25, 1981. It was the most recent in a series of biennial conferences, the previous one having been held in Heidelberg, GFR, in 1979. The series was originated by Richard Laursen, and initially dealt with one aspect of the field, solid-phase sequencing. The scope of the meeting was very broad and among the many aspects of protein sequencing discussed were: instrumentation, strategy, chemicals, mass spectrometry, cleavage of proteins and separation of peptides, and solid, liquid, manual, and even "gas­ phase" sequencing.


ATP ATP-Synthase Amino acid DNA Methodologie Nucleotide RNA enzymes primary structure protein sequence protein structure proteins

Editors and affiliations

  • Marshall Elzinga
    • 1
  1. 1.Brookhaven National LaboratoryUptonUSA

Bibliographic information