In Situ Hybridization Methods

  • Giselbert Hauptmann

Part of the Neuromethods book series (NM, volume 99)

Table of contents

  1. Front Matter
    Pages i-xx
  2. FFPE Sections

    1. Front Matter
      Pages 1-1
    2. Louisa Mezache, Madison Mikhail, Matteo Fassan, Marco Chilosi, Gerard J. Nuovo
      Pages 3-25
  3. Model Organisms

    1. Front Matter
      Pages 27-27
    2. Gina Broitman-Maduro, Morris F. Maduro
      Pages 29-44
    3. José Luis Ferran, Abdelmalik Ayad, Paloma Merchán, Nicanor Morales-Delgado, Luisa Sánchez-Arrones, Antonia Alonso et al.
      Pages 61-82
    4. José Luis Ferran, Abdelmalik Ayad, Paloma Merchán, Nicanor Morales-Delgado, Luisa Sánchez-Arrones, Antonia Alonso et al.
      Pages 83-107
    5. Jong-Hyun Son, Ursula H. Winzer-Serhan
      Pages 109-126
    6. Edgar M. Pera, Helena Acosta, Nadège Gouignard, Maria Climent
      Pages 151-167
  4. Systematic Gene Expression Analysis

  5. Evo-Devo

    1. Front Matter
      Pages 247-247
    2. Yasuhiro Oisi, Osamu Kakitani, Shigeru Kuratani, Kinya G. Ota
      Pages 249-262
    3. Fumiaki Sugahara, Yasunori Murakami, Shigeru Kuratani
      Pages 263-278
    4. Simona Candiani, Greta Garbarino, Mario Pestarino
      Pages 279-292
  6. Neurons, Neuronal Compartments, and Projections

    1. Front Matter
      Pages 319-319
    2. Jacki E. Heraud-Farlow, Tejaswini Sharangdhar, Michael A. Kiebler
      Pages 321-337
    3. Jeremy S. Duncan, Karen L. Elliott, Jennifer Kersigo, Brian Gray, Bernd Fritzsch
      Pages 339-352
  7. miRNA Detection by LNA-FISH

    1. Front Matter
      Pages 353-353
  8. Multiplex Fluorescent In Situ Hybridization (MP-FISH)

    1. Front Matter
      Pages 367-367
    2. Julie Bergalet, Carole Iampietro, Ashley Chin, Xuan-Tam Nguyen, Sulin Oré-Rodriguez, Neal Cody et al.
      Pages 369-391
    3. Hongwei Wang, Nan Su, Li-Chong Wang, Xingyong Wu, Son Bui, Kuang-Jung Chang et al.
      Pages 405-414
  9. Quantum Dot-Based Methods (QD-FISH)

    1. Front Matter
      Pages 415-415
    2. Akira Matsuno, Yudo Ishii, Mineko Murakami, Katsumi Hoya, Shoko M. Yamada, Shinya Miyamoto et al.
      Pages 417-425
    3. Andriani Ioannou, Iro Eleftheriou, Anna Charalambous, Paris A. Skourides
      Pages 451-462
  10. Single Molecule mRNA Detection (SM-FISH)

    1. Front Matter
      Pages 463-463
    2. Remco A. Mentink, Ni Ji, Alexander van Oudenaarden, Hendrik C. Korswagen
      Pages 465-483
  11. Fast FISH

    1. Front Matter
      Pages 539-539
    2. Amaresh K. Ranjan, Mugdha V. Joglekar, Anandwardhan A. Hardikar
      Pages 541-548
  12. Back Matter
    Pages 585-592

About this book


This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.


Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.


TransISH chromogenic RNA ISH techniques fluorescent RNA ISH techniques gene expression genetic model organisms in situ hybridization(ISH) invertebrate multicolor whole-mount ISH (MC-WISH) radioactive and non-radioactive ISH systematic gene expression analysis

Editors and affiliations

  • Giselbert Hauptmann
    • 1
  1. 1.Department of Molecular BiosciencesThe Wenner-Gren Institute, Stockholm UniversityStockholmSweden

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2302-1
  • Online ISBN 978-1-4939-2303-8
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site