Immunocytochemistry and Related Techniques

  • Adalberto Merighi
  • Laura Lossi

Part of the Neuromethods book series (NM, volume 101)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Immunocytochemistry in Non-Mammalian Brain

    1. Front Matter
      Pages 37-37
    2. Nobuhiko Miyasaka, Noriko Wakisaka, Yoshihiro Yoshihara
      Pages 81-92
  3. Detection of Cell Proliferation and Neurogenesis

  4. Identification of Specific Cell Types and Intracellular Pathways

    1. Front Matter
      Pages 151-151
    2. Maria Grazia Bottone, Francesco Paolo Fanizzi, Graziella Bernocchi
      Pages 153-178
    3. Chrystian Junqueira Alves, Alexandre Baldasserini Guimarães, Jessica Ruivo Maximino, Sergio Paulo Bydlowski, Gerson Chadi
      Pages 195-208
    4. Dmitrii E. Korzhevskii, Olga Kirik, Elena Sukhorukova
      Pages 209-224
  5. Quantification of Immunocytochemistry and Combined Techniques

    1. Front Matter
      Pages 255-255
    2. Brad R. Rocco, Kenneth N. Fish
      Pages 257-279
    3. Taisuke Miyazaki, Masahiko Watanabe
      Pages 299-311
    4. Ruth M. Empson, Malinda L. S. Tantirigama, Manfred J. Oswald, Stephanie M. Hughes, Thomas Knöpfel
      Pages 357-373
  6. Novel Approaches

    1. Front Matter
      Pages 375-375
    2. Bas M. C. Cloin, Casper C. Hoogenraad, Marina Mikhaylova, Lukas C. Kapitein
      Pages 389-408
    3. Martin Heubl, Sabine Lévi
      Pages 409-421
    4. Santiago Costantino
      Pages 423-435
  7. Back Matter
    Pages 437-473

About this book


This volume presents a collection of protocols for immunocytochemical analysis of neurons and neural networks. Chapters focus on immunocytochemical localization at light and electronic levels, biochemical characterization, and functional analysis in vivo or ex vivo by novel types of microscopy, as well as protocols for development and production of genetic probes. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. 

Essential and authoritative, Immunocytochemistry and Related Techniques is intended for a large audience of scientists, including histologists, biochemists, cellular and molecular biologists, electrophysiologists that are currently active in the field or are willing to enter the exciting area of neuroscience research.


adult neurogenesis apoptosis array tomography autophagy brain cell proliferation genetic engineering procedures non-mammalian brains protein-patterned substrates tract-tracing techniques

Editors and affiliations

  • Adalberto Merighi
    • 1
  • Laura Lossi
    • 2
  1. 1.Department of Veterinary SciencesUniversity of TorinoGrugliasco, TorinoItaly
  2. 2.Department of Veterinary SciencesUniversity of TorinoGrugliasco, TorinoItaly

Bibliographic information

  • DOI
  • Copyright Information Springer Science+Business Media New York 2015
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-4939-2312-0
  • Online ISBN 978-1-4939-2313-7
  • Series Print ISSN 0893-2336
  • Series Online ISSN 1940-6045
  • Buy this book on publisher's site