Reverse Chemical Genetics

Methods and Protocols

  • Hisashi Koga

Part of the Methods in Molecular Biology™ book series (MIMB, volume 577)

Table of contents

  1. Front Matter
    Pages i-xi
  2. The Importance of Fully-Characterized Genomic Resources and Informatics for Reverse Chemical Genetics

    1. Front Matter
      Pages 1-1
    2. Osamu Ohara
      Pages 3-9
    3. Akihisa Matsuyama, Minoru Yoshida
      Pages 11-24
    4. Wataru Fujibuchi, Hyeryung Kim, Yoshifumi Okada, Takeaki Taniguchi, Hideko Sone
      Pages 55-65
    5. T. S. Keshava Prasad, Kumaran Kandasamy, Akhilesh Pandey
      Pages 67-79
  3. Modification of cDNA Resources Utilized for Reverse Chemical Genetics

  4. The Refinement of Established Methods by Recent Innovations

  5. Potential Applicable Techniques for Reverse Chemical Genetics

    1. Front Matter
      Pages 198-198
    2. Xiaobo Yu, Nicole Schneiderhan-Marra, Hsin-Yun Hsu, Jutta Bachmann, Thomas O. Joos
      Pages 199-214
    3. Toshifumi Shiroya, Hiroyuki Tanaka, Minako Hanasaki, Hisao Takeuchi
      Pages 215-225
    4. Motoki Kyo, Kimihiko Ohtsuka, Eiji Okamoto, Kazuki Inamori
      Pages 227-234
    5. Takeshi Akasaka, Karen Ocorr
      Pages 235-249
  6. Back Matter
    Pages 269-272

About this book


Now a major approach in the identification of certain gene product functions, reverse chemical genetics allows the identification of target genes to precede phenotypic changes created by specific small molecules. In Reverse Chemical Genetics: Methods and Protocols, expert authors introduce fundamental resources, including databases for reverse chemical genetics, as well as the modification of these cDNA resources by the most recent molecular technologies. The book explores newly established methods in addition to potentially applicable techniques that make use of specific small molecules, plus functional assays utilizing these molecules, while focusing on methods that make feasible the functional discovery and comprehensive knowledge of particular target genes. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Reverse Chemical Genetics: Methods and Protocols promises to inspire researchers to conduct their own experiments in order to progress this vital field of study.


2D electrophoresis Escherichia coli In situ hybridization Microarray PCR Small molecules Surface plasmon resonance Target genes cDNA libraries gene expression genes genetics hybridization molecular biology

Editors and affiliations

  • Hisashi Koga
    • 1
  1. 1.Iwai Orthopaedic Medical HospitalTokyoJapan

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2009
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-231-5
  • Online ISBN 978-1-60761-232-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site