Volume 2: Methods and Protocols

  • Donald Armstrong

Part of the Methods in Molecular Biology™ book series (MIMB, volume 580)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Oxidized Lipids

    1. Front Matter
      Pages 1-1
    2. Kenji Matsui, Koichi Sugimoto, Pattana Kakumyan, Sergey A. Khorobrykh, Jun’ichi Mano
      Pages 17-28
    3. Arnis Kuksis, Jukka-Pekka Suomela, Marko Tarvainen, Heikki Kallio
      Pages 39-91
    4. Fan Yi, Si Jin, Pin-Lan Li
      Pages 93-107
    5. Fabio Luiz D’Alexandri, Renata Tonhosolo, Emilia A. Kimura, Alejandro Miguel Katzin
      Pages 109-128
    6. Yulia Y. Tyurina, Vladimir A. Tyurin, Valentina I. Kapralova, Andrew A. Amoscato, Michael W. Epperly, Joel S. Greenberger et al.
      Pages 153-183
  3. Trafficking and Profiling

    1. Front Matter
      Pages 186-186
    2. Françoise Hullin-Matsuda, Reiko Ishitsuka, Miwa Takahashi, Toshihide Kobayashi
      Pages 203-220
    3. Vineet Choudhary, Roger Schneiter
      Pages 221-232
    4. Elsa Arias-Barrau, Concetta C. DiRusso, Paul N. Black
      Pages 233-249
    5. Maximilian Schicher, Iris Jesse, Ruth Birner-Gruenberger
      Pages 251-266
  4. Software and Bioinformatics

    1. Front Matter
      Pages 284-284
    2. Perttu Haimi, Krishna Chaithanya, Ville Kainu, Martin Hermansson, Pentti Somerharju
      Pages 285-294
    3. Josef Cvačka, Edita Kofroňová
      Pages 295-316
    4. Craig E. Wheelock, Susumu Goto, Laxman Yetukuri, Fabio Luiz D’Alexandri, Christian Klukas, Falk Schreiber et al.
      Pages 339-368
  5. Biostatistics

    1. Front Matter
      Pages 370-370
  6. Back Matter
    Pages 383-390

About this book


Essential in biological functions like cell signaling and, when disturbed, a likely cause of disease, lipids have proven to be a vital force in cell biology. In Lipidomics: Methods and Protocols, an international panel of experts present a wide variety of reviewed as well as unpublished data on isolation techniques, structural analysis, lipid rafts, lipid trafficking and profiling, biomarkers, lipid peroxidation, biostatistics applied to lipids, software tools, and bioinformatics. These studies range from simple systems, such as in yeast, to complex biological models. Samples used in these studies include: mitochondria, microsomes, endosomal membranes, RBS and plasma membranes, vesicles, caveolae, liposomes, macrophages, meibum, brain, microglia, retina, adipose tissue, keratinocytes, pulmonary surfactant, and hepatic cultures.  Volume 2 focuses on oxidized lipids, trafficking and profiling, software, bioinformatics, and biostatistics. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include useful introductions to their respective topics, lists of the necessary equipment and materials, step-by-step, readily reproducible laboratory protocols, and authoritative notes on troubleshooting and avoiding common pitfalls.

Comprehensive and cutting-edge, Lipidomics: Methods and Protocols will support researchers who wish to increase their utilization of lipidomics, which will certainly lead to more powerful technology, improved diagnostic-prognostic capabilities, and the identification of new valuable products.


Biostatistics Macrophages Metabolites Metabolomics Oxidized lipids Profiling Software and bioinformatics Trafficking Vivo bioinformatics biological functions liposomes molecular biology

Editors and affiliations

  • Donald Armstrong
    • 1
  1. 1.Mars HillU.S.A.

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2010
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-324-4
  • Online ISBN 978-1-60761-325-1
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site