Engineered Zinc Finger Proteins

Methods and Protocols

  • Joel P. Mackay
  • David J. Segal

Part of the Methods in Molecular Biology book series (MIMB, volume 649)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Engineering Zinc Finger Proteins

    1. Front Matter
      Pages 1-1
    2. Mital S. Bhakta, David J. Segal
      Pages 3-30
    3. Stacey Thibodeau-Beganny, Morgan L. Maeder, J. Keith Joung
      Pages 31-50
    4. Andreu Alibés, Luis Serrano, Alejandro D. Nadra
      Pages 77-88
  3. Artificial Transcription Factors

    1. Front Matter
      Pages 89-89
    2. Kimberley A. Hoeksema, D. Lorne J. Tyrrell
      Pages 97-116
    3. Sabine Stolzenburg, Alan Bilsland, W. Nicol Keith, Marianne G. Rots
      Pages 117-132
    4. Adriana S. Beltran, Pilar Blancafort
      Pages 163-182
    5. Claudio Passananti, Nicoletta Corbi, Annalisa Onori, Maria Grazia Di Certo, Elisabetta Mattei
      Pages 183-206
  4. Zinc Finger Nucleases

    1. Front Matter
      Pages 207-207
    2. Vardit Zeevi, Andriy Tovkach, Tzvi Tzfira
      Pages 209-225
    3. Toni Cathomen, Cem Şöllü
      Pages 227-235
    4. Tatjana I. Cornu, Toni Cathomen
      Pages 237-245
    5. Dmitry Y. Guschin, Adam J. Waite, George E. Katibah, Jeffrey C. Miller, Michael C. Holmes, Edward J. Rebar
      Pages 247-256
    6. Dana Carroll, Kelly J. Beumer, Jonathan K. Trautman
      Pages 271-280
    7. Justin P. Hoshaw, Erica Unger-Wallace, Feng Zhang, Daniel F. Voytas
      Pages 299-313
    8. Andriy Tovkach, Vardit Zeevi, Tzvi Tzfira
      Pages 315-336
    9. Miki Imanishi, Shigeru Negi, Yukio Sugiura
      Pages 337-349
  5. Beyond Transcription and Cleavage

    1. Front Matter
      Pages 351-351
    2. Matthew H. Wilson, Alfred L. George Jr
      Pages 353-363
    3. Jason R. Porter, Sarah H. Lockwood, David J. Segal, Indraneel Ghosh
      Pages 365-382
    4. Beatrice I. Lindhout, Tobias Meckel, Bert J. van der Zaal
      Pages 383-398
    5. Henriette O’Geen, Seth Frietze, Peggy J. Farnham
      Pages 437-455
    6. Nancy C. Horton, Chad K. Park
      Pages 457-477
    7. Josep Font, Joel P. Mackay
      Pages 479-491
  6. Back Matter
    Pages 493-500

About this book


Among the many types of DNA binding domains, C2H2 zinc finger proteins (ZFPs) have proven to be the most malleable for creating custom DNA-binding proteins. In Engineered Zinc Finger Proteins: Methods and Protocols, expert researchers from some of the most active laboratories in this field present detailed methods, guidance, and perspectives. The volume contains sections covering the engineering of ZFPs, methods for the creation, evaluation, and delivery of artificial transcription factors (ATFs), methods for the creation and evaluation of zinc finger nucleases (ZFNs), and a collection of the several applications and assays beyond ATFs and ZFNs, including zinc finger transposases and ChIP-seq methodology amongst other subjects. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Engineered Zinc Finger Proteins: Methods and Protocols aims to aid both seasoned practitioners and new investigators with its vital methods and insights as they seek to create the next generation of engineered ZFPs and applications.


Artificial transcription factors DNA DNA binding domains Designer ZFPs ZFP construction Zinc finger nucleases - ZFNs cloning gene expression gene therapy genes genome molecular biology transcription

Editors and affiliations

  • Joel P. Mackay
    • 1
  • David J. Segal
    • 2
  1. 1.School of Molecular &, Microbial BiosciencesUniversity of SydneySydneyAustralia
  2. 2.Dept. Pharmacology & Genome CenterUniversity of California, DavisDavisUSA

Bibliographic information

  • DOI
  • Copyright Information Springer Science + Business Media, LLC 2010
  • Publisher Name Humana Press, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-60761-752-5
  • Online ISBN 978-1-60761-753-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site