Optical Fluorescence Microscopy

From the Spectral to the Nano Dimension

  • Alberto Diaspro

Table of contents

  1. Front Matter
    Pages i-xii
  2. Franco Quercioli
    Pages 1-36
  3. Shakil Rehman, Naveen K. Balla, Elijah Y. Y. Seng, Colin J. R. Sheppard
    Pages 55-74
  4. Zeno Lavagnino, Francesca Cella Zanacchi, Alberto Diaspro
    Pages 75-84
  5. Isabel Escobar, Emilio Sánchez-Ortiga, Genaro Saavedra, Manuel Martínez-Corral
    Pages 85-99
  6. Emiliano Ronzitti, Giuseppe Vicidomini, Francesca Cella Zanacchi, Alberto Diaspro
    Pages 101-110
  7. Kevin Braeckmans, Hendrik Deschout, Jo Demeester, Stefaan C. De Smedt
    Pages 153-163
  8. M. Collini, L. D’Alfonso, M. Caccia, L. Sironi, M. Panzica, G. Chirico et al.
    Pages 165-181
  9. L. Fusi, E. Brunello, M. Reconditi, R. Elangovan, M. Linari, Y.-B. Sun et al.
    Pages 183-189
  10. Gobind Das, Francesco Gentile, Maria Laura Coluccio, G. Cojoc, Federico Mecarini, Francesco De Angelis et al.
    Pages 191-210
  11. Umberto Fascio, Anna Sartori-Rupp
    Pages 231-238
  12. Back Matter
    Pages 239-244

About this book


In the last decade, fluorescence microscopy has evolved from a classical “retrospective” microscopy approach into an advanced imaging technique that allows the observation of cellular activities in living cells with increased resolution and dimensions. A bright new future has arrived as the nano era has placed a whole new array of tools in the hands of biophysicists who are keen to go deeper into the intricacies of how biological systems work. Following an introduction to the complex world of optical microscopy, this book covers topics such as the concept of white confocal, nonlinear optical microscopy, fluctuation spectroscopies, site-specific labeling of proteins in living cells, imaging molecular physiology using nanosensors, measuring molecular dynamics, muscle braking and stem cell differentiation.


Cellular imaging Fluorescent dyes Nanosensors Spectroscopy Visual system

Editors and affiliations

  • Alberto Diaspro
    • 1
  1. 1., Nanophysics UnitThe Italian Institute of Technology - IIGenovaItaly

Bibliographic information