Amino Acid Analysis Protocols

  • Catherine Cooper
  • Nicolle Packer
  • Keith Williams

Part of the Methods in Molecular Biology™ book series (MIMB, volume 159)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Margaret I. Tyler
    Pages 1-7
  3. Frank D. Macchi, Felicity J. Shen, Rodney G. Keck, Reed J. Harris
    Pages 9-30
  4. Petr Jandik, Christopher Pohl, Victor Barreto, Nebojsa Avdalovic
    Pages 63-85
  5. Noriko Shindo, Tsutomu Fujimura, Saiko Kazuno, Kimie Murayama
    Pages 87-100
  6. Hiroyuki Kataoka, Sayuri Matsumura, Shigeo Yamamoto, Masami Makita
    Pages 101-122
  7. Hiroyuki Kataoka, Norihisa Sakiyama, Yukizo Ueno, Kiyohiko Nakai, Masami Makita
    Pages 183-206
  8. Qingyi Chu, Michael Zeece
    Pages 227-235
  9. Back Matter
    Pages 255-265

About this book


Amino acid analysis is widely used in biotechnology, biomedical, and food analysis laboratories. Amino Acid Analysis Protocols constitutes a major collection of these indispensable analytical techniques, both classic and cutting-edge, of high utility for answering specific biological questions. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Since results depend heavily on the quality of the sample, most contributors have devoted a section to sample preparation, particularly to the collection and storage of bodily fluids. A new method for desalting samples prior to hydrolysis is also provided. Each method is described in step-by-step detail to ensure successful experimental results, and contains helpful notes on pitfalls to avoid, and variations that enable the methods to be used with different systems.
Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Editors and affiliations

  • Catherine Cooper
    • 1
  • Nicolle Packer
    • 1
  • Keith Williams
    • 1
  1. 1.Proteome Systems LtdNorth RydeAustralia

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2000
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-656-7
  • Online ISBN 978-1-59259-047-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site