Sequential Oligopeptide Carriers (SOCn) for Producing Potent Antigens and Effective Immunogens

  • C. Sakarellos
  • V. Tsikaris
  • M. Sakarellos-Daitsiotis
Part of the NATO ASI Series book series (ASEN2, volume 26)


A new class of Sequential Oligopeptide Carriers (SOCn), namely (Lys-Aib-Gly)n (n=2–7), for anchoring antigenic/immunogenic peptides is presented. The aim of this construction was to model artificial carriers with predetermined conformation, so that the attached peptides would not interact to each other or with the carrier, and would thus retain their original active conformation. The NMR study and the molecular dynamics calculations confirmed that the SOCn adopt rigid helicoid structure comparable to a canonical 310-helix, with a somewhat curved axis. The peptides linked to the SOCn retain their original structural profile, as it was also demonstrated by the NMR study. It is concluded that the SOCn impose a favourable disposition of the anchored peptides for generating potent antigens and effective immunogens.


Amide Proton Potent Antigen Multiple Antigenic Peptide Curve Axis Preparative High Performance Liquid Chromatography 
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  1. 1.
    Audibert, F., Jovilet, M., Chedid, L, Amon, R., and Sela, M. (1982) Successful immunization with a totally synthetic diphtheria vaccine, Proc. Natl. Acad. Sci. USA 79, 5042–5046.CrossRefGoogle Scholar
  2. 2.
    Borras-Guesta, F., Fedon, Y., and Petit-Camurden, A. (1988) Enhancement of peptide immunogenicity by linear polymerization, European J. Immunol. 18, 199–202.CrossRefGoogle Scholar
  3. 3.
    Reichlin, M. (1980) Use of glutaraldehyde as a coupling agent for proteins and peptides, Methods Enzymol 70, 159–165.CrossRefGoogle Scholar
  4. 4.
    Liu, F.-T., Zinnecker, M., Hamaoka, T., and Katz, D.H. (1979) New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterization of such conjugates, Biochemistry 18, 690–697.CrossRefGoogle Scholar
  5. 5.
    Bauminger, S. and Wilchek, M. (1980) The use of carbodiimides in the preparation of immunizing conjugates, Methods Biochem. Anal. 70, 151–159.Google Scholar
  6. 6.
    Tam, J. (1988) Synthetic peptide vaccine design: Synthesis and properties of a high-density multiple antigenic peptide system, Proc. Natl. Acad. Sci. USA 85, 5409–5413.CrossRefGoogle Scholar
  7. 7.
    Posnelt, D.N., McGrath, H. and Tarn, J.P. (1988) A novel method for producing anti-peptide antibodies. Production of site-specific antibodies to the T cell antigen receptor β-chain, J. Biol. Chem. 263, 1719–1725.Google Scholar
  8. 8.
    Tarn, J.P., Clavijo, P., Lu, Y.A., Nussenweig, V., Nussenweig, R. and Zavala, F. (1990) Incorporation of T and B epitopes of the circumporozoite protein in a chemically defined synthetic vaccine against Malaria, J. Exp. Med. 171, 299–306.CrossRefGoogle Scholar
  9. 9.
    Briand, J-P., Barin, C., Van Regenmortel, M.H.V. and Muller, S. (1992) Application and limitations of the multiple antigen peptide (MAP) system in the production and evaluation of anti-peptide and anti-protein antibodies, J. Immunol Methods 156, 255–265.CrossRefGoogle Scholar
  10. 10.
    Bindra, V.A. and Kuki, A. (1994) Conformational preferences of oligopeptides rich in α-aminoisobutyric acid. III. Design, synthesis and hydrogen bonding in 310-helices, Int. J. Peptide Protein Res. 44, 539–548.CrossRefGoogle Scholar
  11. 11.
    Tzartos, S.J., Cung, M.T., Demange, P., Loutrari, H., Mamalaki, A., Marraud, M., Papadouli, I., Sakarellos, C., and Tsikaris, V. (1991) The Main Immunogenic Region (MIR) of the Nicotinic Acetylcholine Receptor of the Anti-MIR antibodies, Mol. Neurobiol. 5, 1–29.CrossRefGoogle Scholar
  12. 12.
    Soteriadou, K.P., Remoundos, M.S., Katsikas, M.C., Tzinia, A.K., Tsikaris, V., Sakarellos, C., and Tzartos, S.J. (1992) The Ser-Arg-Tyr-Asp region of the major surface glycoprotein of Leishmania mimics the Arg-Gly-Asp-Ser cell attachment region of Fibronectin, J. Biol. Chem. 267, 13980–13985.Google Scholar
  13. 13.
    James, J.A. and Harley, J.B. (1992) Linear epitope mapping of an Sm B/B polypeptide, J. Immunol. 148, 2074–2079.Google Scholar
  14. 14.
    Tsikaris, V., Sakarellos, C., Cung, M.T., Marraud, M., and Sakarellos-Daitsiotis, M, (1996) Concept and Design of a New Class of Sequential Oligopeptide Carriers (SOC) for Covalent Attachment of Multiple Antigenic Peptides, Biopolymers 38, 291–293.CrossRefGoogle Scholar
  15. 15.
    Wüthrich, K. (1976) Biological Research: Peptides and Proteins, North-Holland, Amsterdam.Google Scholar
  16. 16.
    Tsikaris, V., Detsikas, E., Sakarellos-Daitsiotis, M., Sakarellos, C., Vatzaki, E., Tzartos, S.J., Marraud, M., and Cung, M.T. (1993) Conformational Requirements for Molecular Recognition of Acetylcholine Receptor Main Immunogenic Region (MIR) Analogues by Monoclonal anti-MIR Antibody: A Two-Dimensional Nuclear Magnetic Resonance and Molecular Dynamics Approach, Biopolymers, 33, 1123–1134.CrossRefGoogle Scholar
  17. 17.
    Tsikaris, V., Cung, M.T., Sakarellos, C., Tzinia, A.K., Soteriadou, K.P., and Sakarellos-Daitsiotis, M. (1994) NMR study on a SRYD-containing Fibronectin-like sequence (250-257) of Leishmania gp63: Contribution of residual water in the dimethylsulfoxide solution structure, J. Chem. Soc. Perkin Trans. 2, 821–826.Google Scholar
  18. 18.
    Tsikaris, V., Vlachoyiannopoulos, P.G., Panou-Pomonis, E., Marraud, M., Sakarellos, C., Moutsopoulos, H.M., and Sakarellos-Daitsiotis, M. (1986) Immunoreactivity and conformation of the P-P-G-M-R-P-P repetitive epitope of the Sm autoantigen. Int. J. Peptide Protein Res. in press.Google Scholar
  19. 19.
    Tsikaris, V., Sakarellos, C., Orlewski, P., Marraud, M., Cung, M.T., Vatzaki, E., Tzartos, S., and Sakarellos-Daitsiotis, M. Construction and Application of a New Class of Sequential Oligopeptide Carriers (SOCn) for Multiple Anchoring of Antigenic Peptides. Application to the AChR Main Immunogenic Region. Submitted to Int. J. Biol. Macromol.Google Scholar
  20. 20.
    Tsikaris, V., Sakarellos, C., Marraud, M., Cung, M.T., Tzinia, A., Soteriadou K.P., and Sakarellos-Daitsiotis, M. Application of Sequential Oligopeptide Carriers (SOCn) in protective immunity against Leishmania infection. In preparation.Google Scholar
  21. 21.
    Tsikaris, V., Vlachoyiannopoulos, P.G., Alexopoulos, Ch., Dimitriou, I., Panou-Pomonis, E., Sakarellos C., Moutsopoulos, H.M., and Sakarellos-Daitsiotis, M. Immunological and conformational studies of the major epitope of the Sm autoantigen anchored to Sequential Oligopeptide Carriers (SOCn). In preparation.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1997

Authors and Affiliations

  • C. Sakarellos
    • 1
  • V. Tsikaris
    • 1
  • M. Sakarellos-Daitsiotis
    • 1
  1. 1.Department of ChemistryUniversity of IoanninaIoanninaGreece

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