Cytogenetic Manipulations in Forest Trees Through Tissue Culture

  • S. E. Schlarbaum
Part of the Forestry Sciences book series (FOSC, volume 24-26)


Research conducted in the last ten years has shown exciting possibilities of genetically engineering plants through the developing parasexual procedures in biotechnology (26, 72). Experiments have been conducted on a wide range of plants using approaches ranging from single gene transfer via a plasmid vector (51, 88, 103) to fusion of protoplasts from different phylads (1, 39, 109). The prospect of genetic recombination without a sexual cycle is of considerable interest to forestry as the long term reproductive cycles of trees have often hampered efforts to combine desirable traits in a reasonable period of time. These long reproductive cycles often restrict or preclude certain breeding approaches, e.g. extensive backcrossing, commonly used in agronomic or horticultural crops. Controlled crossing for transfer of a desirable trait(s) in trees is expensive because of the time involved, manpower, often specialized machinery, and field space with associated maintenance. Any technique that could accomplish genetic transfer and bypass the time necessary for experimental material to reach reproductive maturity and the expense associated with conventional tree breeding would be extremely valuable to improvement programs. Moreover, a rapid gene transfer system that could be used in a recurrent fashion may allow for the application of some agronomic or horticultural breeding schemes to forest tree breeding.


Forest Tree Recipient Cell Flow Sorting Genetic Engineering Technique Somatic Cell Genetic 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  • S. E. Schlarbaum

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