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On-the-Fly Fluorescence Lifetime Detection in Capillary Electrophoresis for DNA Analysis

  • Linda B. McGown
Chapter
Part of the Topics in Fluorescence Spectroscopy book series (TIFS, volume 7)

Conclusions

The studies described in this chapter illustrate potential applications of on-the-fly fluorescence lifetime detection in CE for DNA analysis. The combined advantages of speed, high resolution, and small sample volume provided by CE and the accuracy and signal resolution provided by fluorescence lifetime in no more time than is needed for fluorescence intensity detection alone make frequency-domain lifetime detection a promising approach to problems in DNA analysis. Development of more dyes that offer a wider range of lifetimes and reduction in background contributions to the fluorescence signal will help to fully realize the potential of this approach.

Keywords

Capillary Electrophoresis Fluorescence Lifetime Excitation Beam Neutral Density Filter Lifetime Component 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Kluwer Academic Publishers 2003

Authors and Affiliations

  • Linda B. McGown
    • 1
  1. 1.Department of ChemistryDuke UniversityDurham

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