Peptide Nucleic Acids as Agents to Modify Target Gene Expression and Function
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PNA binding-induced transcription to stimulate target gene expression provides an attractive strategy to modify expression of selected genes. This technology could have a broad range of applications including the treatment of many human diseases by inducing expression of therapeutically important genes. PNA binding-induced transcription may also provide a powerful method to study the molecular mechanism of transcription initiation and gene expression regulation. However, the molecular mechanisms of PNA binding-induced transcription need to be elucidated to provide a better understanding of the molecular basis of this important technology. The same is true for the ability of PNA clamps to provoke DNA repair and induce mutagenesis. As these technical issues are resolved, the applications of PNA technology in the treatment of human diseases may become feasible.
KeywordsGreen Fluorescent Protein K562 Cell Green Fluorescent Protein Expression Peptide Nucleic Acid HeLa Nuclear Extract
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