Advertisement

Quality control and standardization of Aloe products

  • Kim Kyeong Ho 
  • Park Jeong Hill 
Chapter
  • 454 Downloads

5.6. Conclusion

Quality control methods for aloe and aloe preparations, which are widely used as ingredients of health foods and cosmetics, were developed. Thirteen phenolic components in Aloe barbadensis and Aloe arborescens were separated and quantified by HPLC. Among them, a quantitative HPLC method for determination of aloesin was developed. For the pharmacokinetic study of aloesin, a new and sensitive HPLC method was developed, as was a new method to detect the adulteration of commercial aloe gel powders.

Keywords

HPLC Chromatogram High Performance Liquid Chromatographic Determination Aloe Barbadensis Aloe Species Aloe Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Biltz JJ, Smith JW, Gerard JR (1963) Aloe vera gel in peptic ulcer theraphy: preliminary report. J Am Osteop Assoc 62. 731Google Scholar
  2. 2.
    Chakkodabylu SR (1989) Sensitive method for the analysis of phospholipid subclasses and molecular species as 1-anthroylnitile derivatives of their diglycerides. JChromatogr 491, 37–48.Google Scholar
  3. 3.
    Conner J, Gray A, Reynolds T, Waterman PG (1989) Anthrone and chromone derivatives in the exudate of Aloe rabaiensis Phytochemistry 28, 3551–3553CrossRefGoogle Scholar
  4. 4.
    Conner JM, Gray AI, Waterman PG, Reynolds T (1990a). Novel anthrone-anthraquinone dimer from Aloe elgonica J Nat Prod 53(5),1362–1364Google Scholar
  5. 5.
    Conner JM, Gray AI, Reynolds T, Waterman PG (1990b) Anthrone and chromone components of Aloe cremnophila and A. jacksonii leaf exudates. Phytochemistry 29(3), 941–944CrossRefGoogle Scholar
  6. 6.
    Crewe JEMD (1985) Aloe in the Treatment of Burns and Scalds Minne sota Medicine 22. 538Google Scholar
  7. 7.
    Fairbairn JW, Simic S (1960) Vegetable Purgatives containing anthracene derivatives. Part XI. Further work on the aloin-like substance of Rhamnus purshiana DC J Pharm Pharmacol 12 45T–51TGoogle Scholar
  8. 8.
    Franz G, Gruen M (1983) Chemistry, Occurrence and Biosynthesis of C-glycosyl Compounds in Plants, Planta Med 47 131–140PubMedGoogle Scholar
  9. 9.
    Gowda DC, Heeiisiddaiah B, Anjaneyalu YU (1979) Structural studies of polysaccharides from Aloe vera Carbohydrate Res 72, 201–205CrossRefGoogle Scholar
  10. 10.
    Goto J, Goto N, Shamsa F, Saito M, Komatzu S, Suzaki K, Nambara T (1983a) New sensitive derivatization of hydroxysteroids for high-performance liquid chromatography with fluorescence detection. Anal Chim Acta 147, 397–400CrossRefGoogle Scholar
  11. 11.
    Goto J, Saito M, Chikai T, Goto N, Nambara T (1983b) Determination of serum bile acids by high-performance liquid chromatography with fluorescence labelling. J Chromatogr 276, 289–300PubMedGoogle Scholar
  12. 12.
    Goto J, Shamsa F, Nambara T (1983c) Determination of 6β-hydroxycortisol in urine by high-performance liquid chromatography with flurescence detection. J Liq Chromatogr 6(11), 1977–1985Google Scholar
  13. 13.
    Graf E, Alexa M (1980) Stability of diastereomeric aloins A and B and their main decomposition product 4-hydroxyaloin Planta Med 38, 121–127Google Scholar
  14. 14.
    Hans WR, Anette B (1993) Thin layer chromatographic screening and high performance liquid chromatographic determination of 5-hydroxyaloin A in the genus aloe. Z Naturforsch 8c, 1Google Scholar
  15. 15.
    Hayness LJ, Holdsworth DK (1970) C-Glycosyl compounds. part. aloesin, a C-glycosyl-chromone from Aloe sp J Chem Soc (C) 74, 2581Google Scholar
  16. 16.
    Haynes LJ, Holdsworth DK, Russell R (1970). C-glycosyl compounds. VI. Aloesin, a C-glycosylchromone from Aloe sp J Chem Soc C, (18), 2581–2586CrossRefGoogle Scholar
  17. 17.
    Heggers JP, Kucukcelebi A, Stabenau CJ, Ko F, Broemeling, Lyle D, Robson, Martin C, Winters, Wendell D (1995) Woundhealing effects of Aloe gel and other topical antibacterial agents on rat skin. Phytother Res 9(6), 455–457Google Scholar
  18. 18.
    Heggers JP, Kucukcelebi A, Stabenau Catherine J, Ko F, Broemeling, Lyle D, Robson, Martuin, C, and Winters Wendell D. (1995) Wound healing effects of Aloe gel and other topical antibacterial agents on rat skin. Phytother Res 9, 455–457Google Scholar
  19. 19.
    Hikino H, Takahashi M, Murakami M, Konno C, Mirin Y, Karikura, M., Hayashi T (1986) Isolation and hypoglycemic activity of arborans A and B, glycans of Aloe arborescens var. natalensis leaves. Int J Crude Drug Res 24(4), 183–186Google Scholar
  20. 20.
    Hirata T, Kushi Y, Suga T, Christensen A (1976) Structural studies of aloenin; the crystal structure of its aglycone. Chem Lett (4), 393–396CrossRefGoogle Scholar
  21. 21.
    Hirata T, Suga T (1977) Biologically active constituents of leaves and roots of Aloe arborescens var. nataliensis. Zeitchirft fur Naturforschung, 32, 731–734Google Scholar
  22. 22.
    Holdsworth DK (1971) Chromones in Aloe species. Part I, Aloesin a C-glucosyl-7-hydroxychromone. Planta Medica 19, 322–325PubMedGoogle Scholar
  23. 23.
    Hodge JE, Hofreiter BT (1962) Determination of reducing sugar carbohydrates. Methods in Carbohydrate Chemistry 1. 380–394Google Scholar
  24. 24.
    Ishii Y, Tanizawa H, Takino Y (1984) Flurophotometry of barbaloin in Aloe, Chem Pharm Bull 32, 4946–4950PubMedGoogle Scholar
  25. 25.
    Kennedy JF, White CA (1983) in Bioactive Carbohydratesin Chemistry, Biochemistry and Biology. Ellis Horwood Limited., Chichester 161Google Scholar
  26. 26.
    Kim KH, Kim HJ, Park JH, Shin YG (1996) Determination of aloesin in aloe preparations by HPLC, Yakhak Hoeji 40, 177–182Google Scholar
  27. 27.
    Lee LM, Haggers JP, Robson MC., Hagstrom WJ. (1980) The therapeutic efficacy of Aloe vera cream in thermal injuries: Two case report. J. Am. Anim.Hosp Assoc 16. 768Google Scholar
  28. 28.
    Makino K, Yagi A, Nishioka I (1974) Constituents of Aloe arborescens var natalensis. III. Structures of two new aloesin ester. Chem Pham Bull 22, 1565–1570Google Scholar
  29. 29.
    Mandel G, Das A (1980) Structure of the D-galactan isolated, from Aloe barbadensis Miller. Carbohydrate Res 86, 247–257CrossRefGoogle Scholar
  30. 30.
    Mandel G, Das A (1980) Structure of the glucoumannan isolated from the leaves of Aloe barbadensis Miller. Carbohydrate Res 87, 249–256CrossRefGoogle Scholar
  31. 31.
    Mebe PP (1987). 2′-p-Methoxycoumaroylaloeresin, a C-glucoside from Aloe excelsa. Phytochemistry 26(9), 2646–2647CrossRefGoogle Scholar
  32. 32.
    Metori A, Ogamo A, Nakagawa Y (1993) Quantitation of monohydroxy fatty acids by high-performance liquid chromatography with fluorescence detection. J. Chromatogr 622, 147–151PubMedGoogle Scholar
  33. 33.
    Morton J (1961). Folk uses and commercial exploitation of Aloe leaf pulp. Economic Botany 15, 311–319Google Scholar
  34. 34.
    Nakamura H, Kan T, Kishimoto K, Ikeda k, Amemiya T, Ito K, Watanabe Y (1989) Gas chromatographic and mass spectrometric determination of aloe components in skin-care cosmetics, Eisei Kagaku 35, 219–225Google Scholar
  35. 35.
    Nakamura H, Okuyuma T (1990) Gas chromatographic and mass spectral determination of aloenin in skin-care cosmetics, J Chromatogr 509, 377–382PubMedCrossRefGoogle Scholar
  36. 36.
    Obata M, Ito S, Beppu H, Fujita K, Nagatsu T (1993). Mechanisim of anti-inflammatory and antithermal burn action of CPase from Aloe arborescens natalensis in rats and mice. Phytother Res 7 (Spec. lssue, Proceedings of the International Congress of Phyto-therapy, 1991). S30–S33Google Scholar
  37. 37.
    Okamura N, Hine N, Harada S, Fujioka T, Mihashi K, Yagi A (1996) Three chromone components form Aloe vera leaves Phytochemistry 43, 495–498CrossRefGoogle Scholar
  38. 38.
    Park MK, Park JH, Kim KH, Shin YG, Myoung KM, Lee JH (1995) Chemical constituents of Aloe capensis Kor J Pharmacog, 26, 244–247Google Scholar
  39. 39.
    Park MK, Park JH, Shin YG, Kim WY, Lee JH, Kim KH (1996) Neoaloesin A: A new C-glucofuranosylchromone from Aloe barbadensis Planta Med 62, 363–365PubMedGoogle Scholar
  40. 40.
    Pelley RP (1992) Aloe quality control: Current status of high pressure liquid chromatography in the quality control of Aloe barbadensis extracts. Aloe Today Autumm 19–26Google Scholar
  41. 41.
    Pelley RP, Wang YT, Waller TA (1993) Current status of quality control of Aloe barbadensis extracts. SOFW Journal April 255–268Google Scholar
  42. 42.
    Rai PP, Turner TD (1975) A method for the estimination of anthraquinones using densitometric thin layer chromatography. J Chromatography 104, 196CrossRefGoogle Scholar
  43. 43.
    Rauwald HW, Beil A (1993a) High-performance liquid chromatographic separation and determination of diastereomeric anthrone-C-glucosyls in Cape aloe J Chromatogr 639, 359–362CrossRefGoogle Scholar
  44. 44.
    Rauwald HW, Beil A (1993b) 5-Hydroxyaloin A in the genus Aloe. Thin layer chromatographic screening and high performance liquid chromatographic determination, Z Naturforsch C: Biosci 48, 1–4Google Scholar
  45. 45.
    Reynolds GW (1950) The Aloes of South Africa, Johan-nesberg, South Africa: The aloes of South Africa Book Fund Google Scholar
  46. 46.
    Reynolds GW (1996) The Aloes of Tropical Africa and Madagascar, Mbabane, Swaziland: The Aloes Book Fund Google Scholar
  47. 47.
    Reynolds T (1985) The compounds in Aloe leaf exudates: a review. Botanical Journal of Linnean Society, 90, 157–177Google Scholar
  48. 48.
    Saito H, Imanishi K, Okabe S (1989) Effects of aloe extract, aloctin A, on gastric secretion and on experimental gastric lesions in rats. Yakugaku Zasshi, 109(5), 335–339PubMedGoogle Scholar
  49. 49.
    (1991) Science and Technical Committee of the IASC, Official Certification Program For Aloe Vera International Aloe Science Council Inc 1–22Google Scholar
  50. 50.
    Speranza G, Dada G, Lunazzi L, Gramatica P, Manitto P (1986a) Studies on Aloe. Part 3. A C-glucosylated 5-methylchromone form Kenya aloe Phytochemistry 25, 2219–2222CrossRefGoogle Scholar
  51. 51.
    Speranza G, Dada G, Lunazzi L, Gramatica P, Manitto P (1986b) Studies on Aloe. Part 4. Aloenin B, a new diglucosylated 6-phenyl-2-pyrone form Kenya aloe, J. Nat. Prod., 49, 800–805CrossRefGoogle Scholar
  52. 52.
    Suzuki Morita T, Haneda M, Ochi K, Shiba M (1986) Determination by high-performance liquid chromatography and identification of barbaloin in aloe, Iyakuhin Kenkyu 17, 984–990Google Scholar
  53. 53.
    Waller TA, Strickland FM, Pelley RP (1994) Quality control and biolobical activity of Aloe Barbadensis extracts useful in the cosmetic industry CTMW 64–80Google Scholar
  54. 54.
    Wawrznowicz T, Hajnos MW, Mulak-Banaszek K (1994) Isolation of aloine and aloeemodine from Aloe (Liliaceae) by micropreparative TLC J Planar Chromatogr-Mod TLC 7, 315–317Google Scholar
  55. 55.
    Whister RL, Wolfrom ML, BeMiller JN, Shafizadeh F (1962) Methods in Carbohydrate Chemistry Volume 1Google Scholar
  56. 56.
    Yagi A, Makino K, Nishioka I (1977) Studies on the constituents of Aloe saponaria Haw. III. The structures of phenol glucosides. Chem Pharm Bull 25(7), 1771–1776Google Scholar
  57. 57.
    Yagi A, Harada N, Shimomura K, Nishioka I (1987) Bradykinin-degrading glycoprotein in Aloe arborescens natalensis Planta Medica 53(1), 19–21PubMedCrossRefGoogle Scholar
  58. 58.
    Yamamoto M, Ishikawa M, Masui T (1985) High performance liquid chromatographic determination of barbaloin in aloe Bull Shizuoka Pref Inst Publ Hlth and Environ Sci 28, 35Google Scholar
  59. 59.
    Yamamoto M, Ishikawa M, Masui T, Nakazawa H, Kabasawa Y (1985) Liquid chromatographic determination of barbaloin(Aloin) in foods J Assoc Off AnalChem 68, 493Google Scholar
  60. 60.
    Yamamoto M, Masui T, Sugiyama K, Yokota M, Nakagomi K, Nakazawa H (1991) Anti-inflammatory active constituents of Aloe arborescens Miller Agri BiolChem 55(6), 1627–1629Google Scholar
  61. 61.
    Yaun A, Kang S, Tan L, Raun B, Fan Y (1991) Isolation and identification of aloesin from the leaves of Aloe vera L var chinensis(Haw.) Berger Zhongguo Ihongyao Zazhi 16(5), 292–293Google Scholar
  62. 62.
    Zonta F, Bogoni P, Masotti P, Micali G (1995) High-performance Liquid Chromatographic profiles of aloe constituents and determination of aloin in beverages, with reference to the EEC regulation for flavoring substances J Chromatogr A 718, 99–106PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2006

Authors and Affiliations

  • Kim Kyeong Ho 
    • 1
  • Park Jeong Hill 
    • 2
  1. 1.College of PharmacyKangwon National UniversityChuncheonKorea
  2. 2.College of PharmacySeoul National UniversitySeoulKorea

Personalised recommendations