Plant Cells pp 63-102 | Cite as

Integrated Bioprocessing for Plant Cell Cultures

  • Jeong-Woo Choi
  • Gyu Heon Cho
  • Sang Yo Byun
  • Dong-Il Kim
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 72)


Plant cell suspension culture has become the focus of much attention as a tool for the production of secondary metabolites including paclitaxel, a well-known anticancer agent. Recently, it has also been regarded as one of the host systems for the production of recombinant proteins. In order to produce phytochemicals using plant cell cultures, efficient processes must be developed with adequate bioreactor design. Most of the plant secondary metabolites are toxic to cells at the high concentrations required during culture. Therefore, if the product could be removed in situ during culture, productivity might be enhanced due to the alleviation of this toxicity. In situ removal or extractive bioconversion of such products can be performed by in situ extraction with various kinds of organic solvents. In situ adsorption using polymeric resins is another possibility. Using the fact that secondary metabolites are generally hydrophobic, various integrated bioprocessing techniques can be designed not only to lower toxicity, but also to enhance productivity. In this article, in situ extraction, in situ adsorption, utilization of cyclodextrins, and the application of aqueous two-phase systems in plant cell cultures are reviewed.


Plant cell culture In situ extraction In situ adsorption Cyclodextrin Aqueous two-phase systems 


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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Jeong-Woo Choi
    • 1
  • Gyu Heon Cho
    • 2
  • Sang Yo Byun
    • 3
  • Dong-Il Kim
    • 4
  1. 1.Department of Chemical EngineeringSogang UniversitySeoulKorea
  2. 2.Department of Chemical EngineeringKangwon National UniversityChunchonKorea
  3. 3.Department of Chemical Engineering and TechnologyAjou UniversitySuwonKorea
  4. 4.Department of Biological Engineering and Center of Advanced Bioseparation TechnologyInha UniversityInchonKorea

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