Molecularly Imprinted Materials — Receptors More Durable than Nature Can Provide

  • Oliver Brüggemann
Part of the Advances in Biochemical Engineering/Biotechnology book series (ABE, volume 76)


The chapter describes the concept of molecular imprinting. This technology allows the fabrication of artificial polymeric receptors applicable in many areas of biotechnology. Polymers imprinted with selected template molecules can be used as specific recognition elements in sensors or as selective stationary phases in affinity chromatography or in capillary electrochromatography. However, also in solid phase extraction or immunoassays these polymers (MIP) are able to compete with traditional materials such as biological antibodies. Furthermore, polymers molecularly imprinted with so-called transition state analogue templates can be applied as catalysts. In other words, these kind of polymers may be used as artificial antibodies (plastibodies) or biomimicking enzymes (plastizymes). Compared to their biological counterparts, MIP offer different advantages such as simplicity in manufacturing and durability. Thus, the author expects MIP to have a major impact on the whole area of biotechnology.


Catalysis Capillary electrochromatography MIP Polymer Sensor Solid phase extraction 


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  1. 1.
    Mosbach K, Ramström O (1996) Bio/Technology 14:163CrossRefGoogle Scholar
  2. 2.
    Wulff G (1995) Angew Chem Int Ed Engl 34:1812CrossRefGoogle Scholar
  3. 3.
    Shea KJ (1994) Trends Polym Sci 2:166Google Scholar
  4. 4.
    Vidyasankar S, Arnold FH (1995) Curr Opin Biotechnol 6:218CrossRefGoogle Scholar
  5. 5.
    Whitcombe MJ, Alexander C, Vulfson EN (1997) Trends Food Sci Technol 8:140CrossRefGoogle Scholar
  6. 6.
    Fischer L, Müller R, Ekberg B, Mosbach K (1991) J Am Chem Soc 113:9358CrossRefGoogle Scholar
  7. 7.
    Andersson LI, Ekberg B, Mosbach K (1993) Bioseparation and catalysis in molecularly imprinted polymers. In: Ngo TT (ed) Molecular interactions in bioseparations. Plenum Press, New York, p 383Google Scholar
  8. 8.
    Kriz D, Ramstrom O, Svensson A, Mosbach K (1995) Anal Chem 67:2142CrossRefGoogle Scholar
  9. 9.
    Siemann M, Andersson LI, Mosbach K (1996) J Agric Food Chem 44:141CrossRefGoogle Scholar
  10. 10.
    Matsui J, Miyoshi Y, Doblhoff-Dier O, Takeuchi T (1995) Anal Chem 67:4404CrossRefGoogle Scholar
  11. 11.
    Sellergren B (1994) J Chromatogr 673:133CrossRefGoogle Scholar
  12. 12.
    Matsui J, Doblhoff-Dier O, Takeuchi T (1995) Chem Lett 6:489CrossRefGoogle Scholar
  13. 13.
    Baggiani C, Trotta F, Giraudi G, Giovannoli C, Vanni A (1999) Anal Commun 36:263CrossRefGoogle Scholar
  14. 14.
    Matsui J, Miyoshi Y, Takeuchi T (1995) Chem Lett 11:1007CrossRefGoogle Scholar
  15. 15.
    Bjarnason B, Chimuka L, Ramström O (1999) Anal Chem 71:2152CrossRefGoogle Scholar
  16. 16.
    Kempe M, Mosbach K (1995) J Chromatogr 691:317CrossRefGoogle Scholar
  17. 17.
    Kempe M, Mosbach K (1995) Tetrahedr Lett 36:3563CrossRefGoogle Scholar
  18. 18.
    Mayes AG, Andersson LI, Mosbach K (1994) Anal Biochem 222:483CrossRefGoogle Scholar
  19. 19.
    Nilsson KGI, Sakguchi K, Gemeiner P, Mosbach K (1995) J Chromatogr 707:199CrossRefGoogle Scholar
  20. 20.
    Sreenivasan K (1997) Polym Int 42:169CrossRefGoogle Scholar
  21. 21.
    Whitcombe M, Rodriguez M, Villar P, Vulfson E (1995) J Am Chem Soc 117:7105CrossRefGoogle Scholar
  22. 22.
    Vidyasankar S, Ru M, Arnold FH (1997) J Chromatogr 775:51CrossRefGoogle Scholar
  23. 23.
    Glad M, Norrlöw O, Sellergren B, Siegbahn N, Mosbach K (1985) J Chromatogr 347:11CrossRefGoogle Scholar
  24. 24.
    Kempe M, Glad M, Mosbach K (1995) J Mol Recognit 8:35CrossRefGoogle Scholar
  25. 25.
    Venton D, Gudipati E (1995) Biochim Biophys Acta 1250:126Google Scholar
  26. 26.
    Spivak DA, Shea KJ (1998) Macromolecules 31:2160CrossRefGoogle Scholar
  27. 27.
    Siemann M, Andersson LI, Mosbach K (1997) J Antibiot 50:88Google Scholar
  28. 28.
    Levi R, McNiven S, Piletsky SA, Cheong SH, Yano K, Karube I (1997) Anal Chem 69:2017CrossRefGoogle Scholar
  29. 29.
    Skudar K, Brüggemann O, Wittelsberger A, Ramström O (1999) Anal Commun 36:327CrossRefGoogle Scholar
  30. 30.
    Brüggemann O, Haupt K, Ye L, Yilmaz E, Mosbach K (2000) J Chromatogr 889:15CrossRefGoogle Scholar
  31. 31.
    Tarbin JA, Sharman M (1999) Anal Commun 36:105CrossRefGoogle Scholar
  32. 32.
    Baggiani C, Giraudi G, Trotta F, Giovannoli C, Vanni A (2000) Talanta 51:71CrossRefGoogle Scholar
  33. 33.
    Rachkov A, McNiven S, El’skaya A, Yano K, Karube I (2000) Anal Chim Acta 405:23CrossRefGoogle Scholar
  34. 34.
    Brüggemann O, Freitag R, Whitcombe MJ, Vulfson EN (1997) J Chromatogr 781:43CrossRefGoogle Scholar
  35. 35.
    Lin JM, Nakagama T, Uchiyama K, Hobo T (1996) Chromatographia 43:585CrossRefGoogle Scholar
  36. 36.
    Walshe M, Garcia E, Howarth J, Smyth MR, Kelly MT (1997) Anal Commun 34:119CrossRefGoogle Scholar
  37. 37.
    Schweitz L, Andersson LI, Nilsson S (1997) J Chromatogr 792:401CrossRefGoogle Scholar
  38. 38.
    Schweitz L, Andersson LI, Nilsson S (1997) Anal Chem 69:1179CrossRefGoogle Scholar
  39. 39.
    Matsui J, Okada M, Tsuruoka M, Takeuchi T (1997) Anal Commun 34:85CrossRefGoogle Scholar
  40. 40.
    Muldoon MT, Stanker LH (1997) Anal Chem 69:803CrossRefGoogle Scholar
  41. 41.
    Matsui J, Fujiwara K, Ugata S, Takeuchi T (2000) J Chromatogr 889:25CrossRefGoogle Scholar
  42. 42.
    Ferrer I, Lanza F, Tolokan A, Horvath V, Sellergren B, Horvai G, Barcelo D (2000) Anal Chem 72:3934CrossRefGoogle Scholar
  43. 43.
    Andersson LI (2000) Analyst 125:1515CrossRefGoogle Scholar
  44. 44.
    Berggren C, Bayoudh S, Sherrington D, Ensing K (2000) J Chromatogr 889:105CrossRefGoogle Scholar
  45. 45.
    Mullett WM, Lai EPC (1998) Anal Chem 70:3636CrossRefGoogle Scholar
  46. 46.
    Zander Å, Findlay P, Renner T, Sellergren B (1998) Anal Chem 70:3304CrossRefGoogle Scholar
  47. 47.
    Piletsky SA, Piletskaya EV, Elgersma AV, Yano K, Karube I, Parhometz YP, Elskaya AV (1995) Biosens Bioelectron 10:959CrossRefGoogle Scholar
  48. 48.
    Sergeyeva TA, Piletsky SA, Brovko AA, Slinchenko EA, Sergeeva LM, Panasyuk TL, El’skaya AV (1999) Analyst 124:331CrossRefGoogle Scholar
  49. 49.
    Piletsky SA, Piletskaya EV, Elskaya AV, Levi R, Yano K, Karube I (1997) Anal Lett 30:445Google Scholar
  50. 50.
    Jakusch M, Janotta M, Mizaikoff B, Mosbach K, Haupt K (1999) Anal Chem 71:4786CrossRefGoogle Scholar
  51. 51.
    Lahav M, Kharitonov AB, Katz O, Kunitake T, Willner I (2001) Anal Chem 73:720CrossRefGoogle Scholar
  52. 52.
    Piletsky SA, Piletskaya EV, Sergeyeva TA, Panasyuk TL, Elskaya AV (1999) Sensors Actuators 60:216CrossRefGoogle Scholar
  53. 53.
    Suarez-Rodriguez JL, Diaz-Garcia ME (2000) Anal Chim Acta 405:67CrossRefGoogle Scholar
  54. 54.
    Chen GH, Guan ZB, Chen CT, Fu LT, Sundaresan V, Arnold FH (1997) Nature Biotechnol 15:354CrossRefGoogle Scholar
  55. 55.
    Malitesta C, Losito I, Zambonin PG (1999) Anal Chem 71:1366CrossRefGoogle Scholar
  56. 56.
    Cao L, Zhou XC, Li SFY (2001) Analyst 126:184CrossRefGoogle Scholar
  57. 57.
    Liang C, Peng H, Bao X, Nie L, Yao S (1999) Analyst 124:1781CrossRefGoogle Scholar
  58. 58.
    Haupt K, Noworyta K, Kutner W (1999) Anal Commun 36:391CrossRefGoogle Scholar
  59. 59.
    Tan Y, Peng H, Liang C, Yao S (2001) Sensors Actuators B 73:179CrossRefGoogle Scholar
  60. 60.
    Dickert FL, Lieberzeit P, Tortschanoff M (2000) Sensors Actuators B 65:186CrossRefGoogle Scholar
  61. 61.
    Dickert FL, Tortschanoff M, Bulst WE, Fischerauer G (1999) Anal Chem 71:4559CrossRefGoogle Scholar
  62. 62.
    Slade C, Vulfson EN (1998) Biotech Bioeng 57:211CrossRefGoogle Scholar
  63. 63.
    Müller R, Andersson LI, Mosbach K (1993) Makromol Chem Rapid Commun 14:637CrossRefGoogle Scholar
  64. 64.
    Brüggemann O (2001) Anal Chim Acta 435:197CrossRefGoogle Scholar
  65. 65.
    Liu X-C, Mosbach K (1998) Macromol Rapid Commun 19:671CrossRefGoogle Scholar
  66. 66.
    Muldoon M, Stanker LJ (1995) Agric Food Chem 43:1424CrossRefGoogle Scholar
  67. 67.
    Haupt K, Mayes AG, Mosbach K (1998) Anal Chem 70:3936CrossRefGoogle Scholar
  68. 68.
    Surugiu I, Danielsson B, Ye L, Mosbach K, Haupt K (2001) Anal Chem 73:487CrossRefGoogle Scholar
  69. 69.
    Asanuma H, Kakazu M, Shibata M, Hishiya T, Komiyama M (1997) Chem Commun 1971Google Scholar
  70. 70.
    Hishiya T, Shibata M, Kakazu M, Asanuma H, Komiyama M (1999) Macromolecules 32:2265CrossRefGoogle Scholar
  71. 71.
    Ye L, Weiss R, Mosbach K (2000) Macromolecules 33:8239CrossRefGoogle Scholar
  72. 72.
    Milojkovic SS, Kostoski D, Comor JJ, Nedeljkovic JM (1997) Polymer 38:2853CrossRefGoogle Scholar
  73. 73.
    Pinel C, Loisil P, Gallezot P (1997) Adv Mater 9:582CrossRefGoogle Scholar
  74. 74.
    Aherne A, Alexander C, Payne MJ, Perez N, Vulfson EN (1996) J Am Chem Soc 118:8771CrossRefGoogle Scholar
  75. 75.
    Lübke C, Lübke M, Whitcombe MJ, Vulfson EN (2000) Macromolecules 33:5098CrossRefGoogle Scholar
  76. 76.
    Piletsky SA, Piletska EV, Chen B, Karim K, Weston D, Barrett G, Lowe P, Turner APF (2000) Anal Chem 72:4381CrossRefGoogle Scholar
  77. 77.
    Ye L, Cormack PAG, Mosbach K (1999) Anal Commun 36:35CrossRefGoogle Scholar
  78. 78.
    Idziak I, Benrebouh A (2000) Analyst 125:1415CrossRefGoogle Scholar
  79. 79.
    Ramström O, Ye L, Krook M, Mosbach K (1998) Anal Commun 35:9CrossRefGoogle Scholar
  80. 80.
    Ye L, Yu Y, Mosbach K (2001) Analyst 126 (Advance article)Google Scholar
  81. 81.
    Ansell RJ, Mosbach K (1998) Analyst 123:1611CrossRefGoogle Scholar
  82. 82.
    Mayes AG, Mosbach K (1996) Anal Chem 68:3769CrossRefGoogle Scholar
  83. 83.
    Piletsky SA, Piletskaya EV, Panasyuk TL, El’skaya AV, Levi R, Karube I, Wulff G (1998) Macromolecules 31:2137CrossRefGoogle Scholar
  84. 84.
    Mathew-Krotz J, Shea KJ (1996) J Am Chem Soc 118:8154CrossRefGoogle Scholar
  85. 85.
    Kobayashi T, Wang HY, Fujii N (1998) Anal Chim Acta 365:81CrossRefGoogle Scholar
  86. 86.
    Wang HY, Kobayashi T, Fukaya T, Fujii N (1997) Langmuir 13:5396CrossRefGoogle Scholar
  87. 87.
    Yoshikawa M, Izumi J, Ooi T, Kitao T, Guiver MD, Robertson GP (1998) Polymer Bull 40:517CrossRefGoogle Scholar
  88. 88.
    Yoshikawa M, Ooi T, Izumi J (1999) J Appl Polym Sci 72:493CrossRefGoogle Scholar
  89. 89.
    Ellwanger A, Owens PK, Karlsson L, Bayoudh S, Cormack P, Sherrington D, Sellergren B (2000) J Chromatogr 897:317CrossRefGoogle Scholar
  90. 90.
    Schweitz L, Andersson LI, Nilsson S (1998) J Chromatogr 817:5CrossRefGoogle Scholar
  91. 91.
    Masque N, Marce RM, Borrull F, Cormack PAG, Sherrington DC (2000) Anal Chem 72:4122CrossRefGoogle Scholar
  92. 92.
    Andersson LI, Paprica A, Arvidsson T (1996) Chromatographia 43:585CrossRefGoogle Scholar
  93. 93.
    Andersson LI, Paprica A, Arvidsson T (1997) Chromatographia 46:57CrossRefGoogle Scholar
  94. 94.
    Andersson LI (2000) J Chromatogr 739:163CrossRefGoogle Scholar
  95. 95.
    Mullett WM, Lai EPC, Sellergren B (1999) Anal Commun 36:217CrossRefGoogle Scholar
  96. 96.
    Andersson L, Mandenius C, Mosbach K (1988) Tetrahedr Lett 29:5437CrossRefGoogle Scholar
  97. 97.
    Andersson LI, Miyabayashi A, O’Shannessy DJ, Mosbach K (1990) J Chromatogr 516:323CrossRefGoogle Scholar
  98. 98.
    Kriz D, Mosbach K (1995) Anal Chim Acta 300:71CrossRefGoogle Scholar
  99. 99.
    Sergeyeva TA, Piletsky SA, Brovko AA, Slinchenko EA, Sergeeva LM, El’skaya AV (1999) Anal Chim Acta 392:105CrossRefGoogle Scholar
  100. 100.
    Kriz D, Kempe M, Mosbach K (1996) Sens Actuators 33:178CrossRefGoogle Scholar
  101. 101.
    Hedborg E, Winquist F, Lundström I, Andersson LI, Mosbach K (1993) Sens Actuators A 37:796CrossRefGoogle Scholar
  102. 102.
    Jenkins AL, Yin R, Jensen JL (2001) Analyst 126 (Advance article)Google Scholar
  103. 103.
    Turkewitsch P, Wandelt B, Darling GD, Powell WS (1998) Anal Chem 70:2025CrossRefGoogle Scholar
  104. 104.
    Ji H-S, McNiven S, Ikebukuro K, Karube I (1999) Anal Chim Acta 390:93CrossRefGoogle Scholar
  105. 105.
    Dickert FL, Hayden O (1999) Fresenius J Anal Chem 364:506CrossRefGoogle Scholar
  106. 106.
    Haupt K, Mosbach K (2000) Chem Rev 100:2495CrossRefGoogle Scholar
  107. 107.
    Stevenson JD, Thomas NR (2000) Nat Prod Rep 17:535CrossRefGoogle Scholar
  108. 108.
    Leonhardt A, Mosbach K (1987) React Polym 6:285Google Scholar
  109. 109.
    Robinson DK, Mosbach K (1989) J Chem Soc Chem Commun 969Google Scholar
  110. 110.
    Ohkubo K, Urata Y, Hirota S, Funakoshi Y, Sagawa T, Usui S, Yoshinaga K (1995) J Mol Catal 101:L111CrossRefGoogle Scholar
  111. 111.
    Sellergren B, Shea KJ (1994) Tetrahedron 5:1403CrossRefGoogle Scholar
  112. 112.
    Beach JV, Shea KJ (1994) J Am Chem Soc 116:379CrossRefGoogle Scholar
  113. 113.
    Ohya Y, Miyaoka J, Ouchi T (1996) Rapid Commun 17:871CrossRefGoogle Scholar
  114. 114.
    Liu X-C, Mosbach K (1997) Macromol Rapid Commun 18:609CrossRefGoogle Scholar
  115. 115.
    Matsui J, Nicholls IA, Karube I, Mosbach K (1996) J Org Chem 61:5414CrossRefGoogle Scholar
  116. 116.
    Polborn K, Severin K (1999) Chem Commun 2481Google Scholar
  117. 117.
    Sellergren B, Karmalkar RN, Shea KJ (2000) J Org Chem 65:4009CrossRefGoogle Scholar
  118. 118.
    Yilmaz E, Mosbach K, Haupt K (1999) Anal Commun 36:167CrossRefGoogle Scholar
  119. 119.
    Takeuchi T, Fukuma D, Matsui J (1999) Anal Chem 71:285CrossRefGoogle Scholar
  120. 120.
    Lanza F, Sellergren B (1999) Anal Chem 71:2092CrossRefGoogle Scholar
  121. 121.
    Sabourin L, Ansell RJ, Mosbach K, Nicholls IA (1998) Anal Commun 35:285CrossRefGoogle Scholar
  122. 122.
    Berglund J, Lindbladh C, Nicholls IA, Mosbach K (1998) Anal Commun 35:3CrossRefGoogle Scholar
  123. 123.
    Dai S, Shin YS, Barnes CE, Toth LM (1997) Chem Mater 9:2521CrossRefGoogle Scholar
  124. 124.
    Katz A, Davis ME (2000) Nature 403:286CrossRefGoogle Scholar
  125. 125.
    Markowitz MA, Kust PR, Deng G, Schoen PE, Dordick JS, Clark DS, Gaber BP (2000) Langmuir16:1759CrossRefGoogle Scholar
  126. 126.
    Lee S-W, Ichinose I, Kunitake T (1998) Langmuir 14:2857CrossRefGoogle Scholar
  127. 127.
    Yano K, Tanabe K, Takeuchi T, Matsui J, Ikebukuro K, Karube I (1998) Anal Chim Acta 363:111CrossRefGoogle Scholar
  128. 128.
    Shi H, Tsai W-B, Garrison MD, Ferrari S, Ratner BD (1999) Nature 398:593CrossRefGoogle Scholar
  129. 129.
    Rachkov A, Minoura N (2001) Biochim Biophys Acta 1544:255Google Scholar
  130. 130.
    Ye L, Ramström O, Ansell RJ, Mansson M-O (1999) Biotech Bioeng 64:650CrossRefGoogle Scholar
  131. 131.
    D’Souza SM, Alexander C, Carr SW, Waller AM, Whitcombe MJ, Vulfson EN (1999) Nature 398:312CrossRefGoogle Scholar
  132. 132.
    Biffis A, Graham NB, Siedlaczek G, Stalberg S, Wulff G (2001) Macromol Chem Phys 202:163CrossRefGoogle Scholar
  133. 133.
    Marty J-D, Tizra M, Mauzac M, Rico-Lattes I, Lattes A (1999) Macromolecules 32:8674CrossRefGoogle Scholar
  134. 134.
    Perez N, Whitcombe MJ, Vulfson EN (2001) Macromolecules 34:830CrossRefGoogle Scholar
  135. 135.
    Yilmaz E, Haupt K, Mosbach K (2000) Angew Chem 112:2178CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Oliver Brüggemann
    • 1
  1. 1.Institut für ChemieTechnische Universität BerlinBerlinGermany

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