Advertisement

Strategies for the Isolation of Antigen-specific B Lymphocytes

  • Richard P. Phipps
Chapter

Abstract

Since the 1960s a number of methods have been devised to purify and subsequently to study populations of lymphocytes with known antigen specificity (Haas and Layton, 1975; Scott, 1976; Snow et al., 1983a; Phipps et al., 1986a). Although techniques have been developed to purify both antigen-specific B and T lymphocytes (Haas and Von Boehmer, 1978; Basch et al., 1983), this review is restricted to an examination of recent strategies for the isolation of antigen-specific B lymphocytes.

Keywords

Spleen Cell Human Monoclonal Antibody Tetanus Toxoid Magnetic Microsphere Plastic Bead 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aldo-Benson, M. A. (1986). An in vitro model for clonal anergy in continuously growing antigen-specific B cell lines. Cell. Immunol. 101, 391–402.CrossRefPubMedGoogle Scholar
  2. Barald, K. F. and Wessels, N. K. (1984). Differential antigen adhesivity used to select spleen cells for the production of monoclonal antibodies to embryonic neurons. J. Immunol. Methods 73, 1–15.CrossRefPubMedGoogle Scholar
  3. Basch, R. S., Berman, J. W. and Lakow, E. (1983). Cell separation using positive immunoselective techniques. J. Immunol. Methods 56, 269–280.CrossRefPubMedGoogle Scholar
  4. Bonner, W. A., Hulett, H., Sweet, R. and Herzenberg, L. A. (1972). Fluorescence activated cell sorting. Rev. Sci. Instrum. 43, 404.CrossRefPubMedGoogle Scholar
  5. Braun, J. (1983). Spontaneous in vitro occurrence and long-term culture of murine B lymphoblast cell lines. J. Immunol. 130, 2113–2116.PubMedGoogle Scholar
  6. Cambier, J. C. and Neale, M. J. (1982). Isolated phosphorylcholine binding lymphocytes. I. Use of a cleavable cross linking reagent for solid-phase adsorbent isolation of functional antigen-binding cells. J. Immunol. Methods 51, 209–221.CrossRefPubMedGoogle Scholar
  7. Cammisuli, S. and Wofsy, L. (1976). Hapten-sandwich labelling III. Bifunctional reagents for immunospecific labelling of cell surface antigens. J. Immunol. 117, 1695–1704.PubMedGoogle Scholar
  8. Casali, P., Inghirami, G., Nakamura, M., Davies, T. F. and Notkins, A. (1986). Human monoclonals from antigen-specific selection of B lymphocytes and transformation by EBV. Science 234, 476–479.CrossRefPubMedGoogle Scholar
  9. Charriere, J., Dardenne, M. and Bach, J. F. (1973). Antigen recognition by T lymphocytes. IV. Difference in antigen-binding characteristics of T and B-RFC: a cause for variations in the evaluation of T-RFC. Cell. Immunol. 9, 32–44.CrossRefGoogle Scholar
  10. Choi, T., Sleigh, D. and Nisonoff, A. (1974). General method for isolation and recovery of B cells bearing specific receptors. J. Exp. Med. 139, 761–766.PubMedCentralCrossRefPubMedGoogle Scholar
  11. Clark, N. W. T., Parkhouse, R. and Simmonds, R. G. (1982). Positive and negative selection of cells by hapten-modified antibodies. J. Immunol. Methods 51, 167–170.CrossRefPubMedGoogle Scholar
  12. Cuatracasas, P. and Anfinsen, C. B. (1971). Affinity chromatography. Ann. Rev. Biochem. 40, 259–278.CrossRefGoogle Scholar
  13. Dangl, J. L. and Herzenberg, L. A. (1982). Selection of hybridomas and hybridoma variants using the fluorescence activated cell sorter. J. Immunol. Methods 52, 1–14.CrossRefPubMedGoogle Scholar
  14. Eady, R. P., Hough, D. W., Kilshaw, P. J. and Stesvenson, G. T. (1974). Recovery of immunoglobin removed from lymphocytic surfaces by proteolysis. Immunology 26, 549–561.PubMedCentralPubMedGoogle Scholar
  15. Elliott, B. E. (1979). In: Lefkovitz, I. and Pernis, B. (eds.), Immunological Methods 1, 241–259.Google Scholar
  16. Elliott, B. E. and Haskill, J. S. (1973). Characterization of thymus-derived and bone marrow derived rosette-forming lymphocytes. Eur. J. Immunol. 3, 68–74.CrossRefPubMedGoogle Scholar
  17. Gaudernack, G., Leivestad, T., Ugelstad, J. and Thorsby, E. (1986). Isolation of pure functionally active CD8+ T cells. Positive selection with monoclonal antibodies directly conjugated to monosized magnetic microspheres. J. Immunol. Methods 90, 179–187.CrossRefPubMedGoogle Scholar
  18. Haas, W. (1975). Separation of antigen-specific lymphocytes. II. Enrichment of hapten-specific antibody-forming cell precursors. J. Exp. Med. 141, 1015–1029.CrossRefPubMedGoogle Scholar
  19. Haas, W. and Layton, J. (1975). Separation of antigen-specific lymphocytes. I. Enrichment of antigen-binding cells. J. Exp. Med. 141, 1004–1014.CrossRefPubMedGoogle Scholar
  20. Haas, W., Schrader, J. W. and Szenberg, A. (1974). A new simple method for the preparation of lymphocytes bearing specific receptors. Eur. J. Immunol. 4, 565–577.CrossRefPubMedGoogle Scholar
  21. Haas. W. and Von Boehmer, H. (1978). Techniques for separation and selection of antigen-specific lymphocytes. Curr. Topics Micro. Immunol. 84, 1–120.Google Scholar
  22. Iverson, I. G. (1973). Long-lived B memory cells separated on antigen coated bead columns. Nature New Biol. 243, 23–24.Google Scholar
  23. Julius, M. H. and Herzenberg, L. A. (1974). Isolation of antigen binding cells from unprimed mice. Demonstration of antibody-forming cell precursor activity and correlation between precursor and secreted antibody avidities. J. Exp Med. 140, 904–920.PubMedCentralCrossRefPubMedGoogle Scholar
  24. Kenny, J. J., Merrill, J. E. and Ashman, R. S. (1978). A two step centrifugation procedure for the purification of sheep erythrocyte antigen-binding cells. J. Immunol. 120, 1233–1239.PubMedGoogle Scholar
  25. Klinman, N. R. (1972). The mechanism of primary and secondary clonal precursor cells. J. Exp. Med. 136, 241–260.PubMedCentralCrossRefPubMedGoogle Scholar
  26. Kozbor, D. and Roder, J. R. (1981). Requirements for the establishment of high-titred human monoclonal antibodies against tetanus toxoid using the Epstein-Barr virus technique. J. Immunol. 127, 1275–1280.PubMedGoogle Scholar
  27. Mage, M. G., McHugh, L. L. and Rothstein, T. L. (1977). Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. J. Immunol. Methods 15, 47–56.CrossRefPubMedGoogle Scholar
  28. Manderino, G. L., Gooch, G. T. and Stavitsky, A. B. (1978). Preparation, characterization and functions of rabbit lymph node cell populations. I. Preparation of KLH primed T and B memory cells with Anti-Fab’ affinity columns. Cell Immunol. 41, 264–275.CrossRefPubMedGoogle Scholar
  29. Molday, R. S. and MacKenzie, D. (1982). Immunospecific ferromagnetic iron-dextran reagents for the labelling and magnetic separation of cells. J. Immunol. Methods 52, 353–367.CrossRefPubMedGoogle Scholar
  30. Molday, R. S., Yen, S. and Rembaum, A. (1977). Application of magnetic microspheres in labelling and separation of cells. Nature 268, 437–441.CrossRefPubMedGoogle Scholar
  31. Myers, C. D., Sanders, V. M. and Vitetta, E. S. (1986). Isolation of antigen-binding virgin and memory B cells. J. Immunol. Methods 92, 45–57.CrossRefPubMedGoogle Scholar
  32. Nash, A. D. and Boyle, W. (1986). Depletion by monolayer binding of specific precursors of antibody-forming cells directed against cellular antigens. Immunology 57, 411–417.PubMedCentralPubMedGoogle Scholar
  33. Nossal, G. J. V. and Pike, B. (1978). Improved procedures for the fractionation and in vitro stimulation of hapten-specific B lymphocytes. J. Immunol. 120, 145–150.PubMedGoogle Scholar
  34. Nossal, G. J. V., Pike, B. and Battye, F. (1978). Sequential use of hapten-gelatin fractionation and fluorescence-activated cell sorting in the enrichment of hapten-specific B lymphocytes. Eur. J. Immunol. 8, 151–157.CrossRefPubMedGoogle Scholar
  35. Owen, C. S., Mahesh-babu, U., Cohen, S. W. and Maurer, P. H. (1982). Magnetic enrichment of antibody-secreting cells. J. Immunol. Methods 51, 171–181.CrossRefPubMedGoogle Scholar
  36. Phipps, R. P., Pillai, P. S. and Scott, D. W. (1984). Conversion of a tolerogenic to an immunogenic signal by a lymphoid dendritic cell-like tumor line. J. Immunol. 132, 2273–2278.PubMedGoogle Scholar
  37. Phipps, R. P., Szakos, J. and Illig, K. (1986a). A method for the isolation of autoreactive B cells. J. Immunol. Methods 88, 163–170.CrossRefPubMedGoogle Scholar
  38. Phipps, R. P., Baecher, C., Schad, V., Freiinger, J. and Szakos, J. (1986b). Ability to trigger guanosine-specific B lymphocytes in vitro using DNA as antigen. Arth. Rheum. 29, 139 (Abst.).Google Scholar
  39. Pike, B., Vaux, D. L., Clark-Lewis, I., Schrader, J. and Nossal, G. J. V. (1982). Proliferation and differentiation of single hapten-specific B lymphocytes is promoted by T-cell factor(s) distinct from T-cell growth factor. Proc. Natl. Acad. Sci. 79, 6350–6354.PubMedCentralCrossRefPubMedGoogle Scholar
  40. Pike, B., Raubitschek, A. and Nossal, G. J. V. (1984). Human interleukin 2 can promote the growth and differentiation of single hapten-specific B cells in the presence of specific antigen. Proc. Natl. Acad. Sci. 81, 7917–7921.PubMedCentralCrossRefPubMedGoogle Scholar
  41. Pike, B. and Nossal, G. J. V. (1985). A high efficiency cloning system for single hapten-specific B lymphocytes that is suitable for assay of putative growth and differentiation factors. Proc. Natl. Acad. Sci. 82, 3395–3399.PubMedCentralCrossRefPubMedGoogle Scholar
  42. Pillai, P. S. and Scott, D. W. (1981). Hapten-specific murine colony-forming B cells II. Delineation of a tolerogen-sensitive subpopulation of colony-forming B cells. J. Immunol. 127, 1603–1606.PubMedGoogle Scholar
  43. Pillai, P. S., Scott, D. W., White, D. and Corley, R. B. (1984). Major histocompatibility complex-restricted and unrestricted interactions in the T cell-dependent activation of hapten-binding B cells. Immunobiol. 166, 345–359.CrossRefGoogle Scholar
  44. Rubin, B. (1976). Regulation of helper cell activity by specifically absorbable T lymphocytes. J. Immunol. 116, 80–85.PubMedGoogle Scholar
  45. Schlossman, S. and Hudson, L. (1973). Specific purification of lymphocyte populations on a digestable immunoabsorbent. J. Immunol. 110, 313–315.PubMedGoogle Scholar
  46. Scott, D. W. (1976). Antifluorescein affinity columns. Isolation and immunocompetence of lymphocytes that bind fluoresceinated antigens in vivo or in vitro. J. Exp. Med. 144, 69–78.CrossRefPubMedGoogle Scholar
  47. Scott, D. W., Venkataraman, M. and Jandinski, J. (1979). Multiple pathways of B lymphocyte tolerance. Immunological Rev. 43, 241–280.CrossRefGoogle Scholar
  48. Scott, D. W., Livnat, D., Penneil, C. A. and Keng, P. (1986). Lymphoma models for B cell activation and tolerance. III. Cell cycle dependence for negative signalling of WEHI-231 B lymphoma cells by anti-u. J. Exp. Med. 164, 156–164.CrossRefPubMedGoogle Scholar
  49. Snow, E. C., Vitetta, E. S. and Uhr, J. W. (1983a). Activation of antigen-enriched B cells. I. Purification and response to thymus-independent antigens. J. Immunol. 130, 607–613.PubMedGoogle Scholar
  50. Snow, E. L., Noelle, R., Uhr, J. and Vitetta, E. (1983b). Activation of antigen-enriched B cells. II. Role of linked recognition in B cell proliferation to thymus-dependent antigens. J. Immunol. 130, 614–618.PubMedGoogle Scholar
  51. Stein, P., Dubois, P., Greenblatt, D and Howard, M. (1986). Induction of antigen-specific proliferation in affinity-purified small B lymphocytes: requirement for BSF-1 by type 2 but not type 1 thymus-independent antigens. J. Immunol. 136, 2080–2089.PubMedGoogle Scholar
  52. Trelaven, J. G., Gibson, F. M., Ugelstad, J., Rembaum, A., Philip, T. and Kemshead, J. T. (1984). Removal of neuroblastoma cells from bone marrow with monoclonal antibodies conjugated to magnetic microspheres. Lancet 1, 70–73.CrossRefGoogle Scholar
  53. Warner, N. L., Daley, M. J., Richey, J. and Spellman, C. (1979). Flow cytometry analysis of murine B cell lymphoma differentiation. Immunological Rev. 48, 197–244.CrossRefGoogle Scholar
  54. Wigzell, H. (1976). Specific affinity fractionation of lymphocytes using glass or plastic bead columns. Scand. J. Immunol. 5, 23–30.CrossRefPubMedGoogle Scholar
  55. Wigzell, H. and Andersson, B. (1969). Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells. J. Exp. Med. 129, 23–36.PubMedCentralCrossRefPubMedGoogle Scholar
  56. Wigzell, H., Sundquist, K. G. and Yoshida, T. O. (1972). Separation of cells according to surface antigens by the use of antibody-coated columns. Fractionation of cells carrying immunoglobulins and blood group antigens. Scand. J. Immunol. 1, 75–87.CrossRefPubMedGoogle Scholar
  57. Wofsy, L. and Kimura, J. (1971). Cell separation on affinity columns: the preparation of pure populations of anti-hapten specific lymphocytes. J. Immunol. 107, 725–729.PubMedGoogle Scholar
  58. Wofsy, L., Henry, C. and Cammisuli, S. (1978). Hapten-sandwich labelling of cell-surface antigens. Contemp. Top. Mol. Immunol. 7, 215–237.CrossRefPubMedGoogle Scholar
  59. Yefenof, E., Sanders, V., Snow, E. C., Noelle, R. J., Oliver, K. G., Uhr, J. and Vitetta, E. S. (1985). Preparation and analysis of antigen-specific memory B cells. J. Immunol. 135, 3777–3784.PubMedGoogle Scholar

Copyright information

© The Editor and Contributors 1989

Authors and Affiliations

  • Richard P. Phipps

There are no affiliations available

Personalised recommendations