Strategies for the Isolation of Antigen-specific B Lymphocytes
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Since the 1960s a number of methods have been devised to purify and subsequently to study populations of lymphocytes with known antigen specificity (Haas and Layton, 1975; Scott, 1976; Snow et al., 1983a; Phipps et al., 1986a). Although techniques have been developed to purify both antigen-specific B and T lymphocytes (Haas and Von Boehmer, 1978; Basch et al., 1983), this review is restricted to an examination of recent strategies for the isolation of antigen-specific B lymphocytes.
KeywordsSpleen Cell Human Monoclonal Antibody Tetanus Toxoid Magnetic Microsphere Plastic Bead
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