Immunoenzymatic Stainings in Diagnostic Virology
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The laboratory diagnosis of viral infections has been traditionally accomplished by the growth, isolation and identification of the infecting virus. However, it has long been realized that such isolation is a technically demanding and time-consuming technique. The need for a rapid diagnosis of viral diseases has led to the development of other techniques such as (a) serological methods, (b) the detection of virally induced antigens in cell cultures or in tissue sections suspected of harbouring the virus and (c) hybridization techniques able to detect viral DNA in infected cells using labelled probes.
KeywordsRespiratory Syncytial Virus Bovine Leukaemia Virus Herpes Simplex Virus Infection African Swine Fever Immunoperoxidase Technique
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- Brigati, D. J., Myerson, D., Leary, J. J., Spalholz, B., Travis, S. Z., Fong, C. K. Y., Hsiung, G. D. and Ward, D. C. (1983). Detection of viral genomes in cultured cells and paraffin embedded tissue sections using biotin-labelled hybridization probes. Virology, 126, 32–50.PubMedCrossRefGoogle Scholar
- Das, S. K., Sarkar, P. and Sen, G. P. (1985). Evaluation of immunoperoxidase test in routine diagnosis of rabies in animals. Indian J. Anim. Sci., 55, 979–982.Google Scholar
- Gerber, M. A., Shah, K. V., Thung, S. N. and Zu Rhein, G. M. (1980). Immunohistochemical demonstration of common antigen of polyomaviruses in routine histologic tissue sections of animals and man. Am. J. Clin. Pathol., 73, 794–797.Google Scholar
- Graham, D. Y. and Estes, M. K. (1980). In Bricout, F. and Scherrer, R. (eds.), Viral Enteritis in Humans and Animals, INSERM, Paris, pp. 345–348.Google Scholar
- Granlund, D. J., Levine, P. H. and Fuccillo, D. A. (1979). Enzyme immunoassay for detection of antibody to Epstein-Barr virus-specific early antigens. J. Clin. Microbiol., 10, 747–751.Google Scholar
- Hanon, N., Simpson, J. and Eckert, H. L. (1975). Assessment of virus infectivity by the immunofluorescent and immunoperoxidase technique. J. Clin. Microbiol., 1, 324–329.Google Scholar
- Johnson, F. B., Leavitt, R. W. and Richards, D. F. (1985). Comparison of the Scott Selecticult-HSV Kit with conventional culture and direct immunoperoxidase staining for detection of herpes simplex virus in cultures of clinical specimens. J. Clin. Microbiol., 21, 438–441.PubMedCentralPubMedGoogle Scholar
- Moseley, R. C., Corey, L., Benjamin, D., Winter, C. and Remington, M. L. (1981). Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital herpes simplex virus infection. J. Clin. Microbiol., 13, 913–918.PubMedCentralPubMedGoogle Scholar
- Rai, A. and Prasad, I. J. (1980). Immunoperoxidase technique in a rapid detection of foot and mouth disease virus. J. Anim. Sci., 50, 957–960.Google Scholar
- Rijntjes, P. J. M., Van Ditzhuijsen, T. J. M., Van Loon, A. M., Van Haelst, U. J. G. M., Bronkhorst, F. B. and Yap, S. H. (1985). Hepatitis B virus DNA detected in formalin fixed liver specimens and its relation to serologic markers and histopathologic features in chronic liver disease. Am. J. Pathol., 120, 411–418.PubMedCentralPubMedGoogle Scholar