A Novel Homogeneous β-Galactosidase Immunoassay System

  • Dan Monroe


A new generation of enzyme immunoassays (EIAs) that offer better accuracy is now possible with the help of recombinant DNA techniques. The use of fragmented β-galactosidase proteins obtained from genetically engineered Escherichia coli forms the basis of this new concept in EIAs. Traditionally, EIAs have been performed using whole enzyme molecules covalently linked to an antibody, an antigen, or a hapten, as described elsewhere (Monroe, 1983, 1984, 1985). Assay speed and precision are often compromised with conventional methods, however, owing to required sample pretreatment, incubation, and washing steps. Such tedious procedures can now be eliminated by means of this new separation-free or homogeneous β-galactosidase immunoassay system known as CEDIA™ (Henderson et al., 1986).


Tetrameric Enzyme Enzyme Peptide Sodium Potassium Phosphate Homogeneous Immunoassay Enzyme Donor 
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  1. Bertani, L. E. and Bertani, G. (1970). Preparation and characterization of temperate, non-inducible bacteriophage P2 (host; E. coli). J. Gen. Virol., 6, 201–212.CrossRefPubMedGoogle Scholar
  2. Davis, R. W., Botstein, D. and Roth, J. R. (1980). In A Manual for Genetic Engineering, Advanced Bacterial Genetics, Cold Spring Harbor Laboratory, New York, p. 250.Google Scholar
  3. Henderson, D. R., Friedman, S. B., Harris, J. D., Manning, W. B. and Zoccoll, M. A. (1986). CEDIA™, a new homogeneous immunoassay system. Clin. Chem., 32(9), 1637–1641.PubMedGoogle Scholar
  4. Monroe, D. (1983). ELISA: a versatile chemical tool. American Clinical Products Review, 2(3), 22–27.Google Scholar
  5. Monroe, D. (1984). Enzyme immunoassay. Anal. Chem., 56(8), 921A–931A.Google Scholar
  6. Monroe, D. (1985). The solid-phase enzyme-linked immunospot assay: current and potential applications. Biotechniques, 3(3), 222–229.Google Scholar
  7. Steers, E., Jr., Cuatrecasas, P. and Pollard, H. B. (1971). The purification of galactosidase from Escherichia coli by affinity chromatography. J. Biol. Chem., 246, 196–200.PubMedGoogle Scholar
  8. Yokoyama, Y., Shioiri, T. and Yamada, S. (1977). Phosphorus in organic synthesis. XVI. Diphenyl phosphorazidate (DPPA) and diethyl phosphorocyanide (DEPC). Two new reagents for the preparation of thiol esters from carboxylic acids and thiols. Chem. Pharm. Bull., 25, 2423–2429.CrossRefGoogle Scholar

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© The Editor and Contributors 1989

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  • Dan Monroe

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