Interpolation in Assay Systems with a Sigmoid Response Curve
- 34 Downloads
A procedure to facilitate interpolation of results from sigmoid curves, e.g. immunoradiometric assays, radioimmunoassay, titration curves, dose-response curves, is presented. The procedure allows curve fitting even where the zero and/or 100% values are unknown. A minimum and a maximum value are found by the program with pairs of abscissa and ordinate values as the only input information. The data may be weighted. In addition, the program provides information about the point of inflection of the sigmoid curve in order to estimate a 50% response value, which is not always provided in other program-fitting sigmoid curve procedures.
KeywordsTitration Curve Polynomial Regression Sigmoid Curve Immunoradiometric Assay Statistical Quality Control
Unable to display preview. Download preview PDF.
- Berkson, J. (1944). Application of the logistic function to bioassays. J. Amer. Statis. Assoc. 39, 357–365.Google Scholar
- Finney, D. J. (1971). Probit Analysis, 3rd edition, Cambridge University Press.Google Scholar
- Finney, D. J. (1978). Statistical Methods in Biological Assays, 3rd edition, Charles Griffin and Co. Ltd., London.Google Scholar
- Jensen, K. and Wirth, N. (1978). Pascal, User Manual and Report, 2nd edition, Springer Verlag, New York.Google Scholar
- Jordan-Engeln, G. and Reutter, F. (1981). Formelsammlung zur Numerischen Mathematik, 3rd edition, B. I. Hochschultaschenbuecher vol. 106, Bibliographisches Institut, Mannheim. Neider, J. A. and Mead, R. (1965). Computer J. 7, 308–313.Google Scholar