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Tissue Culture Gel Firmness: Measurement And Effects On Growth

  • Stewart I. Cameron
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Part of the Focus on Biotechnology book series (FOBI, volume 6)

Keywords

Petri Plate Tissue Culture Medium Globe Artichoke Digital Force Gauge Somatic Embryogenic Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. [1]
    Horner, M.; McComb, J.A.; McComb, A.J. and Street, H.E. (1977) Ethylene production and plantlet formation by Nicotiana anthers cultured in the presence and absence of charcoal. J. Exp. Bot. 28: 13651372.CrossRefGoogle Scholar
  2. [2]
    Wetzstein, H.Y.; Kim, C. and Sommer, H.E. (1994) Vessel volume, gelling agent, and basal salts affect pH and gel strength of autoclaved tissue culture media. Hort Sci. 29:683-685.CrossRefGoogle Scholar
  3. [3]
    Huang, L.-C.; Kohashi, C.; Vangundy, R. and Murashige, T. (1995) Effects of common components on hardness of culture media prepared with Gelrite™. In Vitro Cell Dev. Biol.-Plant 31: 84-89.CrossRefGoogle Scholar
  4. [4]
    Klimaszewska, K. and Smith, D.R. (1997) Maturation of somatic embryos of Pinus strobusis promoted by a high concentration of gellan gum. Physiol. Plant. 100: 949-957.CrossRefGoogle Scholar
  5. [5]
    Romberger, J.A. and Tabor, C.A. (1971) The Picea abies shoot apical meristem in culture. I. Agar and autoclaving effects. Amer. J. Bot. 58: 131-140.CrossRefGoogle Scholar
  6. [6]
    Brown, D.C.W.; Leung, D.W.M. and Thorpe, T.A. (1979) Osmotic requirement for shoot formation in tobacco callus. Physiol. Plant. 46: 36-41.CrossRefGoogle Scholar
  7. [7]
    von Arnold, S. (1987) Improved efficiency of somatic embryogenesis in mature embryos of Picea abies(L.) Karst. J.Plant Physiol. 128: 233-244.CrossRefGoogle Scholar
  8. [8]
    Li, X.Y.; Huang, F.H. and Gbur, E.E., Jr. (1998) Effect of basal medium, growth regulators and Phytagel concentration on initiation of embryogenic cultures from immature zygotic embryos of loblolly pine (Pinus taeda L.) Plant Cell Rep.17: 298-301.CrossRefGoogle Scholar
  9. [9]
    Bornmann, C.H. and Vogelmann, T.C. (1984) Effect of rigidity of gel medium on benzyladenine-induced adventitious bud formation and vitrification in vitro in Pice abies. Physiol. Plant. 61: 505-512.CrossRefGoogle Scholar
  10. [10]
    Klimaszewska, K.; Bernier-Cardou, M.; Cyr, D.R. and Sutton, B.C.S. (2000) Influence of gelling agents on culture medium gel strength, water availability, tissue water potential, and maturation response in embryogenic cultures of Pinus strobus L. In Vitro Cell. Dev. Biol.–Plant 36: 279-286.CrossRefGoogle Scholar
  11. [11]
    Pasqualetto, P.-L.; Zimmerman, R.H. and Fordham, I. (1988) The influence of cation and gelling agent concentrations on vitrification of apple cultivars in vitro. Plant Cell Tissue Org. Cult. 14:31-40.CrossRefGoogle Scholar
  12. [12]
    Nairn, B.J.; Furneaux, R.H. and Stevenson, T.T. (1995) Identification of an agar constituent responsible for hydric control in micropropagation of radiata pine. Plant Cell Tissue Org. Cult. 43: 1-11.CrossRefGoogle Scholar
  13. [13]
    Marga, F.; Vebret, L. and Morvan, H. (1997) Agar fractions could protect apple shoots cultured in liquid media against hyperhydricity. Plant Cell Tissue Org. Cult. 49: 1-5.CrossRefGoogle Scholar
  14. [14]
    Owens, L.D. and Wozniak, C.A. (1991) Measurement and effects of gel matric potential ad expressibility on production of morphogenic callus by cultured sugarbeet leaf discs. Plant Cell Tissue Org. Cult. 26: 127-133.CrossRefGoogle Scholar
  15. [15]
    Singha, S.; Townsend, E.C. and Oberly, G.H. (1985) Mineral nutrient status of crabapple and pear shoots cultured in vitro on varying concentrations of three agars. Amer. J. Hort. Sci. 110: 407-411.Google Scholar
  16. [16]
    Stecchini, M.L.; Del Torre, M.; Sarais, I.; Saro, O.; Messina, M. and Maltini, E. (1998) Influence of structural properties and kinetic constraints on Bacillus cereus growth. Appl. Environ. Microbiol. 64: 1075-1078.CrossRefGoogle Scholar
  17. [17]
    Debergh, P.C. (1982) Physical properties of culture media. In: Fujiwara, A. (Ed.) Proc. of the 5thInternational Congress of Plant Tissue and Cell Culture. Tokyo, Japan; pp. 135-136.Google Scholar
  18. [18]
    Debergh, P.C.; Harbaoui, Y. and Lemeur, R. (1981) Mass propagation of globe artichoke (Cynara scolymus): Evaluation of different hypotheses to overcome vitrification with special reference to water potential. Physiol. Plant. 53: 81-87.CrossRefGoogle Scholar
  19. [19]
    Spomer, L.A. and Smith, M.A.L. (1996) Direct measurement of water availability in gelled plant tissue culture media. In Vitro Cell. Dev. Biol. - Plant 32: 210-215.CrossRefGoogle Scholar
  20. [20]
    Debergh, P.C. (1983) Effects of agar brand and concentration on the tissue culture medium. Physiol. Plant. 59: 270-276.CrossRefGoogle Scholar
  21. [21]
    Beruto, D.; Beruto, M.; Ciccarelli, C. and Debergh, P. (1995) Matric potential evaluations and measurements for gelled substrates. Physiol. Plant. 94: 151-157.CrossRefGoogle Scholar
  22. [22]
    Whyte, J.N.C.; Englar, J.R. and Hosford, S.P.C. (1984) Factors affecting texture profile evaluation of agar gels. Botanica Marina 27: 63-69.CrossRefGoogle Scholar
  23. [23]
    Cameron, S.I. (2001) Use of a prototype gel tester to demonstrate the effect of variable calcium concentration on gel rigidity. In Vitro Cell. Dev. Biol.- Plant 37: 419-424.CrossRefGoogle Scholar
  24. [24]
    Mao, R.; Tang, J. and Swanson, B.G. (2001) Water holding capacity and microstructure of gellan gels. Carbohydrate Polymers 46: 365-371.CrossRefGoogle Scholar
  25. [25]
    Sarma, K.S., Maesato, K.; Hara, T. and Sonoda, T. (1990) Effect of method of agar addition on post-autoclave pH of the tissue culture media. Ann. Bot. 65: 37-40.CrossRefGoogle Scholar
  26. [26]
    Owen, H.R., Wengerd, D., and Miller, A.R. (1991) Culture medium is influenced by basal medium, carbohydrate source, gelling agent, activated charcoal, and medium storage method. Plant Cell Rep.10: 583-586.CrossRefGoogle Scholar
  27. [27]
    Skirvin, R.M.; Chu, M.C.; Mann, M.L.; Young, H.; Sullivan, J. and Fermanian, T. (1986) Stability of tissue culture medium pH as a function of autoclaving, time, and cultured plant material. Plant Cell Rep. 5: 292-294.CrossRefGoogle Scholar
  28. [28]
    Selby, C., Lee, R., and Harvey, B.M.R. (1989) The effects of culture medium rigidity on adventitious bud production and tissue vitrification in needle cultures of Sitka spruce [Picea sitchensis(Bong.) Carr.] New Phytol. 113: 203-210.CrossRefGoogle Scholar
  29. [29]
    McNaught, A.D. and Wilkinson, A. (1997) IUPAC Compendium of Chemical Technology 2nd ed. Blackwell Science, Oxford, UK; pp. 464. Online at: http://www.nicmila.org/ Gold/Output/S06227.xhtml.Google Scholar
  30. [30]
    Krieg, N.R. and Gerhardt, P. (1981) Solid culture. In: Gerhardt, P.; Murray, R.G.E.; Costilow, R.N.; Nester, E.W.; Wood, W.A.; Krieg, N.R. and Phillips, G.B. (Eds.) Manual of Methods for General Bacteriology. Amer. Soc. For Microbiol., Washington DC; pp. 143-150.Google Scholar
  31. [31]
    Scherer, P.A.; Muller, E.; Lippert, H. and Wolff, G. (1988) Multi-element analysis of agar and gelrite impurities investigated by inductively coupled plasma emission spectrometry as well as physical properties of tissue culture media prepared with agar or the gellan gum Gelrite. Acta Hort. 226: 655-658.CrossRefGoogle Scholar

Copyright information

© Springer 2008

Authors and Affiliations

  • Stewart I. Cameron
    • 1
  1. 1.Natural Resources Canada, Canadian Forest Service-Atlantic Forestry CentreFrederictonCanada

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