Advertisement

Molecular Basis of Transabdominal—A Sexually Dimorphic Mutant of the Bithorax Complex of Drosophila

(cis regulation/macromutation/homeotic/Abdominal B/evolution)
  • Susan E. Celniker
  • E. B. Lewis
Chapter
  • 202 Downloads

Abstract

Transabdominal (Tab) is a dominant gain-of-function mutation that results in islands of sexually dimorphic abdominal cuticle in the dorsal thorax of the adult fly. This phenotype has complete penetrance and constant expressivity, and we show that it results from ectopic expression of ABD-BII, one of two proteins derived from the Abdominal B (Abd-B) domain of the bithorax complex (BX-C) and one that is normally expressed only in terminal portions of the abdomen. In Tab/+ animals ABD-BII is ectopically expressed in the relevant imaginal “wing” disc as three islands of cells whose location on the fate map corresponds to the three islands of transformed cuticle in each half of the adult thorax. Tab is associated with an inseparable inversion bringing sequences in 90E next to sequences in the transcription unit encoding ABD-BII in 89E. That 90E sequences drive ectopic expression of ABD-BII is indicated by our finding that such sequences in a P-element transformant express the reporter gene’s product (β-galactosidase) in the same three islands of wing disc cells. On morphological grounds, the transformed islands in the adult thorax correspond to subsets of muscle attachment cells. Ectopic expression of a homeodomain protein thus creates a unique and invariant pattern of sexual dimorphism.

Keywords

Disc Cell Bithorax Complex Dorsal Thorax Posterior Abdomen Adult Thorax 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Lewis E. B. (1978) Nature (London) 276, 565–570.Google Scholar
  2. 2.
    Sanchez-Herrero E., Vernos I., Marco R., & Morata G, (1985) Nature (London) 313, 108–113.Google Scholar
  3. 3.
    Duncan I. (1987) Annu. Rev. Genet. 21, 285–319.Google Scholar
  4. 4.
    Celniker S. E., Keelan D. J., & Lewis E. B. (1989) Genes Dev. 3, 1425–1437.Google Scholar
  5. 5.
    Scott M. P., Tamkun J. W., & Harzell G. W. (1989) Biochim. Biophys. Acta 989, 25–48.Google Scholar
  6. 6.
    Celniker S. E. & Lewis E. B. (1987) Genes Dev. 1, 111–123.Google Scholar
  7. 7.
    Tautz D. & Pfeifle C. (1989) Chromosoma 98, 81–85.Google Scholar
  8. 8.
    DeLorenzi M., Ali N., Saari G., Henry C., Wilcox M., & Bienz M. (1988) EMBO J. 7, 3233–3231.Google Scholar
  9. 9.
    Kuziora M. A. & McGinnis W. (1988) EMBO J. 7, 3233–3244.Google Scholar
  10. 10.
    Karch F., Weiffenbach B., Peifer M., Bender W., Duncan I., Celniker S., Crosby M., & Lewis E. B. (1985) Cell 43, 81–96.Google Scholar
  11. 11.
    Celniker S. E., Sharma S., Keelan D., & Lewis E. B. (1990) EMBO J. 9, 4277–4286.Google Scholar
  12. 12.
    Zavortink M. & Sakonju S. (1989) Genes Dev. 3, 1969–1981.Google Scholar
  13. 13.
    Bellen H., O’Kane C., Wilson C., Grossniklaur U., Pearson R. K., & Gehring W. J. (1989) Genes Dev. 3, 1288–1300.Google Scholar
  14. 14.
    Bryant P. J. (1975) J. Exp. Zool. 193, 49–78.Google Scholar
  15. 15.
    DeLorenzi M. & Bienz M. (1990) Development 108, 323–329.Google Scholar
  16. 16.
    Miller A. (1950) in Biology of Drosophila, ed. Demerec M. (pmWiley New York), pp. 368–419.Google Scholar
  17. 17.
    Boulet A. M., Lloyd A., & Sakonju S. (1991) Development 111, 393–405.Google Scholar
  18. 18.
    Martinez-Arias A. & Lawrence P. A. (1985) Nature (London) 313, 639–642.Google Scholar
  19. 19.
    Sakonju S., Lewis E. B., & Hogness D. (1984) Genetics 107, s93.Google Scholar
  20. 20.
    Sanchez-Herrero E. & Crosby M. A. (1988) EMBO J. 7, 2163–2173.Google Scholar
  21. 21.
    Duncan I. (1982) Genetics 100, s20.Google Scholar
  22. 22.
    Lipshitz H. (1991) Curr. Opin. Cell Biol. 3, 966–975.Google Scholar
  23. 23.
    St. Johnston D., Beuchle D., & Nusslein-Volhard C. (1991) Cell 66, 51–63.Google Scholar
  24. 24.
    Kuhn D., Woods D. F., & Andrew D. J. (1981) Genetics 99, 99–107.Google Scholar
  25. 25.
    Awad A. A. M., Gausz J., Gyurkovics H., & Parducz A. (1981) Acta Biol. Acad. Sci. Hung. 32, 219–228.Google Scholar
  26. 26.
    King M. & Wilson A. C. (1975) Science 188, 107–116.Google Scholar

Copyright information

© Springer Science+Business Media New York 2004

Authors and Affiliations

  • Susan E. Celniker
    • 1
  • E. B. Lewis
    • 1
  1. 1.Division of BiologyCalifornia Institute of TechnologyPasadenaUSA

Personalised recommendations