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Isolation of Magnetospirillum magneticum AMB-1 Mutants Defective in Bacterial Magnetic Particle Synthesis by Transposon Mutagenesis

  • Aris Tri Wahyudi
  • Haruko Takeyama
  • Tadashi Matsunaga
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Part of the ABAB Symposium book series (ABAB)

Abstract

Nonmagnetic mutants of Magnetospirillum magneticum AMB-1 were recovered following mini-Tn5 transposon mutagenesis. Transconjugants with kanamycin resistance were obtained at a frequency of 2.7 × 10−7 per recipient. Of 3327 transconjugants, 62 were defective for bacterial magnetic particle (BMP) synthesis. The frequency of independent transposition events for nonmagnetic mutants was about 1.4% in transconjugants. Further analy- sis of DNA sequences flanking transposon by inverted polymerase chain reaction allowed isolation of at least 10 genes or DNA sequences involved in BMP synthesis in M. magneticum AMB-1.

Index Entries

Transposon mutagenesis mini-Tn5 Magnetospirillum magneticum AMB-1 inverse polymerase chain reaction bacterial magnetic particles sequence analysis 

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Aris Tri Wahyudi
    • 1
  • Haruko Takeyama
    • 1
  • Tadashi Matsunaga
    • 1
  1. 1.Department of BiotechnologyTokyo University of Agriculture and TechnologyKoganei, TokyoJapan

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