Production of Polyhydroxybutyrate by Bacillus Species Isolated from Municipal Activated Sludge

  • Kin-Ho Law
  • Yun-Chung Leung
  • Hugh Lawford
  • Hong Chua
  • Wai-Hung Lo
  • Peter Hoifu Yu
Part of the ABAB Symposium book series (ABAB)


Plastic wastes are considered to be severe environmental contaminants causing waste disposal problems. Widespread use of biodegradable plastics is one of the solutions, but it is limited by high production cost. Biologic wastewater treatment generates large quantities of biomass as activated sludge. Only a few reports focus on the potential of utilizing resident Bacillus species from activated sludge in polyhydroxbutyrate (PHB) production as well as the production of PHB from food wastes. They have attractive properties such as short generation time, absence of endotoxins, and secretion of both amylases and proteinases that can well utilize food wastes for nutrients, which can further reduce the cost of production of polyhydroxyalkanoates (PHAs). Two PHA-producing strains, HF-1 and HF-2, were isolated from activated sludge. HF-1 outperformed HF-2 in terms of growth and PHB production in hydrolyzed soy and malt wastes. The isolated bacteria was characterized by DNA sequence alignment. Cell extracts of HF-1 were also compared to Bacillus megaterium cell extracts on sodium dodecyl sulfate Polyacrylamide gel electrophoresis. The biopolymers accumulated were analyzed by gas chromatography, nuclear magnetic resonance, and Fourier transform infrared methods.

Index Entries

Polyhydroxybutyrates malt waste soy waste Bacillus activated sludge Fourier transform infrared inclusion body nuclear magnetic resonance 


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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Kin-Ho Law
    • 1
  • Yun-Chung Leung
    • 1
  • Hugh Lawford
    • 2
  • Hong Chua
    • 3
  • Wai-Hung Lo
    • 1
  • Peter Hoifu Yu
    • 1
  1. 1.Department of Applied Biology and Chemical TechnologyHong Kong Polytechnic UniversityHung Hom, Hong KongChina
  2. 2.C Department of BiochemistryUniversity of TorontoTorontoCanada
  3. 3.Civil and Structural EngineeringHong Kong Polytechnic UniversityHung Hom, Hong KongChina

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