Advertisement

Fingerprinting Trichoderma reesei Hydrolases in a Commercial Cellulase Preparation

  • T. B. Vinzant
  • W. S. Adney
  • S. R. Decker
  • J. O. Baker
  • M. T. Kinter
  • N. E. Sherman
  • J. W. Fox
  • M. E. HimmelEmail author
Chapter
Part of the ABAB Symposium book series (ABAB)

Abstract

Polysaccharide degrading enzymes from commercial T. reesei broth have been subjected to “fingerprint” analysis by high-resolution 2-D gel electro-phoresis. Forty-five spots from 11 × 25 cm Pharmacia gels have been analyzed by LC-MS/MS and the resulting peptide sequences were compared to existing databases. Understanding the roles and relationships of compo-nent enzymes from the T. reesei cellulase system acting on complex sub-strates is key to the development of efficient artificial cellulase systems for the conversion of lignocellulosic biomass to sugars. These studies suggestfollow-on work comparing induced and noninduced T. reesei cells at the proteome level, which may elucidate substrate-specific gene regulation and response.

Index Entries

Cellulase Trichoderma reesei two-dimensional gel electro-phoresis liquid chromatography-mass spectrometry/mass spectrometry 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Wyman, C. E., Bain, R. L., Hinman, N. D., and Stevens, D. J. (1993), Renewable Energy:Sources for Fuels and Electricity, Island Press, Washington, DC.Google Scholar
  2. 2.
    Sheehan, J. J. (1994), in Enzymatic Conversion of Biomass for Fuels Production, vol. 566, Himmel, M. E., Baker, J. O., and Overend, R. P., eds., American Chemical Society, Washington, DC, pp. 1–52.CrossRefGoogle Scholar
  3. 3.
    Bergeron, P. (1996), in Handbook on Bioethanol, Wyman, C. E., ed., Taylor & Francis, Washington, DC, pp. 179–195.Google Scholar
  4. 4.
    Swiss-Prot site <http://www.expasy.ch/sprot/>.
  5. 5.
    Herbert, B. R., Sanchez, J.-C, and Bini, L. (1997), in Proteonome Research: New Frontiersin Functional Genomics, Wilkins, M. R., Williams, K. L., Appel, R. D., and Hochstrasser, D. F., eds., Springer Verlag, NY, pp. 13–30.CrossRefGoogle Scholar
  6. 6.
    Schmidt, C. S. and Wolf, G. A. (1999), Eur. J. Plant Pathol. 105, 285–295.CrossRefGoogle Scholar
  7. 7.
    Pakula, T. M., Uusitalo, J., Saloheimo, M., Salonen, K., Aarts, R. J., and Penttila, M.(2000), Microbiology-UK 146, 223–232.Google Scholar
  8. 8.
    Maras, M., De Bruyn, A., Vervecken, W., Uusitalo, J., Penttila, M., Busson, R., Herdewijn, P., and Contreras, R. (1999), FEBS Lett. 452, 365–370.CrossRefGoogle Scholar
  9. 9.
    Saloheimo, M., Nakari-Setala, T., Tenkanen, M., and Penttila, M. (1997), Eur. J.Biochem. 249, 584–591.CrossRefGoogle Scholar
  10. 10.
    Barnett, C. C., Berka, R. M., and Fowler, T. (1991), BioTechnology 9, 562–567.CrossRefGoogle Scholar
  11. 11.
    Margolles-Clark, E., Saloheimo, M., Siika-aho, M., and Penttila, M. (1996), Gene 172, 171, 172.Google Scholar
  12. 12.
    Margolles-Clark, E., Tenkanen, M., Nakari-Setala, T., and Penttila, M. (1996), Appl.Environ. Microbiol. 62, 3840–3846.Google Scholar
  13. 13.
    Margolles-Clark, E., Tenkanen, M., Soderlund, H., and Penttila, M. (1996), Eur. J. Biochem. 237, 553–560.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • T. B. Vinzant
    • 1
  • W. S. Adney
    • 1
  • S. R. Decker
    • 1
  • J. O. Baker
    • 1
  • M. T. Kinter
    • 2
  • N. E. Sherman
    • 2
  • J. W. Fox
    • 2
  • M. E. Himmel
    • 1
    Email author
  1. 1.Biotechnology Center for Fuels and ChemicalsNational Renewable Energy LaboratoryGoldenUSA
  2. 2.Biomolecular Research FacilityUniversity of Virginia Medical SchoolCharlottesvilleUSA

Personalised recommendations