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Development of High-Performance and Rapid Immunoassay for Model Food Allergen Lysozyme Using Antibody-Conjugated Bacterial Magnetic Particles and Fully Automated System

  • Reiko Sato
  • Haruko Takeyama
  • Tsuyoshi Tanaka
  • Tadashi MatsunagaEmail author
Chapter
  • 363 Downloads
Part of the ABAB Symposium book series (ABAB)

Abstract

A high-performance and rapid chemiluminescence immunoassay for model food allergen lysozyme, one of the major allergenic components inegg white, using antibody-conjugated bacterial magnetic particles and a fully automated system was developed. This system contains a reaction station, tip rack, and an eight-tip pipettor that is able to attach and detach a strong magnet to the tip surface. The immunoreaction time was shortened to5 min, and the assay was completed within 20 min. The lower detection limit for lysozyme was 10 ng/mL. This system can be used to perform 24 samples in 60 min within 10% coefficient of variation.

Index Entries

Sandwich immunoassay food allergen lysozyme bacterial magnetic particles fully automated system 

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Reiko Sato
    • 1
  • Haruko Takeyama
    • 1
  • Tsuyoshi Tanaka
    • 1
  • Tadashi Matsunaga
    • 1
    Email author
  1. 1.Department of BiotechnologyTokyo University of Agriculture and TechnologyTokyoJapan

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