Hydrogen Secretion Regulated by Intracellular Membrane Potential: A Hypothesis on the Regulation of Renal Acidification

  • H. Oberleithner
  • W. Wang
Part of the Developments in Nephrology book series (DINE, volume 18)


The question was addressed whether the cell membrane potential could regulate net acid secretion in the kidney. Cells from the amphibian diluting segment were isolated and fused to giant cells. From the evaluation of the intracellular pH-recovery after an acute acid load we postulate an electroneutral Na+/H+ exchange and a rheogenic HCO 3 - transport system in the plasma membrane of these fused cells. Furosemide-induced hyperpolarization of the intracellular membrane potential acidifies the cell cytosol and the tubule fluid. Potassium-induced depolarization does the opposite. We conclude that the cell membrane potential regulates urinary acid secretion.


Giant Cell Renal Epithelial Cell Cell Membrane Potential Cell Cytosol Tubule Fluid 
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Copyright information

© Martinus Nijhoff Publishing, Boston 1987

Authors and Affiliations

  • H. Oberleithner
    • 1
  • W. Wang
    • 1
  1. 1.Department of PhysiologyUniversity Würzburg Medical SchoolWurzburgGermany

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