Hydrogen Secretion Regulated by Intracellular Membrane Potential: A Hypothesis on the Regulation of Renal Acidification
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The question was addressed whether the cell membrane potential could regulate net acid secretion in the kidney. Cells from the amphibian diluting segment were isolated and fused to giant cells. From the evaluation of the intracellular pH-recovery after an acute acid load we postulate an electroneutral Na+/H+ exchange and a rheogenic HCO 3 - transport system in the plasma membrane of these fused cells. Furosemide-induced hyperpolarization of the intracellular membrane potential acidifies the cell cytosol and the tubule fluid. Potassium-induced depolarization does the opposite. We conclude that the cell membrane potential regulates urinary acid secretion.
KeywordsGiant Cell Renal Epithelial Cell Cell Membrane Potential Cell Cytosol Tubule Fluid
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