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Purine degradative enzymes in the malignant cells of patients with B-cell leukemia

  • A. D. Ho
  • B. Dörken
  • W. Hunstein
  • A. V. Hoffbrand
Chapter
  • 42 Downloads
Part of the Developments in Oncology book series (DION, volume 32)

Abstract

The purine degradative enzymes adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP) and ecto-5’-nucleotidase (5-NT) seem to play an important role in the development of both T- and B-lymphocytes [1–4]. Inherited deficiencies of ADA and PNP are associated with various immune defects. Furthermore remarkable differences in enzyme activities of these purine enzymes and terminal deoxynucleotidyl transferase (TdT) have been reported among immunologic subclasses of acute lymphocytic leukemia (ALL) [5–8]. Studies of these enzymes in normal T-cell precursors have shown that different thymic subsets in different stages of maturation are characterized by specific enzyme patterns with a fall in TdT and ADA activities and a rise in PNP and 5’NT activities during T-cell maturation [9, 10]. Further investigations have shown that the enzyme patterns of malignant T-cells are comparable to the enzymatic make up of normal T-lymphocytes in defined stages of development [11, 12]. For example, the blasts of T-ALL have high levels of TdT and ADA activities but low levels of PNP and 5’NT, an enzyme pattern which corresponds to that of prothymocytes, their normal counterparts.

Keywords

Terminal Deoxynucleotidyl Transferase Purine Nucleoside Phosphorylase Enzyme Pattern Prolymphocytic Leukemia Enzyme Terminal Deoxynucleotidyl Transferase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Martinus Nijhoff Publishers, Boston 1985

Authors and Affiliations

  • A. D. Ho
    • 1
  • B. Dörken
    • 1
  • W. Hunstein
    • 1
  • A. V. Hoffbrand
    • 2
  1. 1.Medizinische Universitäts-PoliklinikHeidelbergGermany
  2. 2.Department of HaematologyRoyal Free Hospital and School of MedicineLondonUK

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