Mutagenicity Testing of Complex Environmental Mixtures with Chinese Hamster Ovary Cells
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Because of the positive correlation between mutagenicity and carcinogenicity, short-term tests for mutagenicity are used widely as initial screening tests for substances of potential carcinogenicity. The Ames test (Ames et al., 1975) quantitating the reversion of histidine-requiring Salmonella typhimurium auxotrophs to histidine-nonrequiring prototrophs, has been used in different laboratories to study chemical mixtures obtained through extraction of environmental pollutants. A wide data base obtained from the Ames test includes studies of urban air particles (Pitts et al., 1977), automobile exhaust (Huisingh et al., 1978; Ohnishi et al., 1980; Clark et al., 1981), and coal combustion products (Fisher et al., 1979; Clark and Hobbs, 1980). Ames test-directed chemical fractionation has been used as an approach to identify mutagens in complex environmental mixtures (Dutcher et al., 1981).
KeywordsCHINESE Hamster Ovary Cell Automobile Exhaust Ames Test Exhaust Particle Mutation Assay
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- Ames, B.N., J. McCann, and E. Yamasaki. 1975. Methods for detecting carcinogens and mutagens with the Salmonella/-mammalian-microsome mutagenicity test. Mutation Res. 31:347–364.Google Scholar
- Dutcher, J.S., R.E. Royer, J.O. Hill, C.E. Mitchell, and C.R. Clark. 1981. (in press). Identification of components of biologically active fractions of low BTU gasifier coal tars. Presented at the Chemical Characterization of Hazardous Substance in Synfuels Symposium, Seattle, WA.Google Scholar
- Hanson, R.L., T.R. Henderson, R.L. Carpenter, C.R. Clark, and C.H. Hobbs, T.M. Harvey, and D.F. Hunt. (MS). Identification of mutagens in coal combustion fly ash extracts with gas chromatograph-mass spectrometry (GC/MS) and MS/MS.Google Scholar
- Hsie, A.W., D.B. Couch, J.P. O’Neill, J.R. San Sebastion, P.A. Brimer, R. Machanoff, J.C. Riddle, A.P. Li, J.C. Fuscoe N. Forbes, and M.H. Hsie. 1979. Utilization of a quantitative mammalian cell mutation system CHO/HGPRT, in experimental mutagenesis and genetic toxicology. In: Strategies for Short-Term Testing for Mutagens/Carcinogens. B.E. Butterwork, ed. CRC Press: West Palm Beach, pp. 39–54.Google Scholar
- Huisingh, J., R. Bradow, R. Jungers, L. Claxton, R. Zweidinger, S. Tejada, J. Bumgarner, F. Duffield, M. Waters, V.F. Simmon C. Hare, C. Rodriguez, and L. Snow. 1978. Application of bioassay to the characterization of diesel particle emissions. In: Application of Short-Term Bioassays in the Fractionation and Analysis of Complex Environmental Mixtures M. Waters, S. Nesnow, J. Huisingh, S. Sandhu, and L. Claxton eds. EPA 600/9–78–027. U.S. Environmental Protection Agency, Research Triangle Park, NC. pp. 1–32.Google Scholar
- Huisingh, J.L., R.L. Bradow, R.H. Jungers, B.D. Harris, R.B. Zweidinger, K.M. Cushing, B.E. Gill, and R.E. Albert. 1980. Mutagenic and carcinogenic potency of extracts of diesel and related environmental emissions: Study design, sample generation, collection and preparation. In: Health Effects of Diesel Engine Emissions: Proceedings of an International Symposium, Volume 2. W.E. Pepelko, R.M. Danner, and N.A. Clarke, eds. EPA-600/9–80–057b. pp. 788–800.Google Scholar
- Li, A.P. 1981b. Simplification of the CHO/HGPRT mutation assay through the growth of Chinese hamster ovary cells as unattached cultures. Mutation Res. 85:165–175.Google Scholar
- Li, A.P. (in press). Quantitation of mutation at the hypoxanthine-guanine phosphoribosyl transferase and Na+ -K+-ATPase gene loci in Chinese hamster ovary cells. J. Tissue Culture Methods.Google Scholar
- Li, A.P., C.R. Clark, R.L. Hanson, T.R. Henderson, and C.H. Hobbs. (MS). Comparative mutagenicity of a coal-combustion fly ash extract in Salmonella typhimurium and Chinese hamster ovary cells.Google Scholar
- Li, A.P. and R. Shimizu. (MS). Increasing the sensitivity of CHO/HGPRT mutation assay through increasing sampling size.Google Scholar