A Quantitative, Clonal Assay for Carcinogen-Induced Alterations of Respiratory Epithelial Cells in Culture

  • J. Carl Barrett
  • Thomas E. Gray
  • Marc J. Mass
  • David G. Thomassen
Part of the Environmental Science Research book series (ESRH, volume 27)


The identification of environmental carcinogens and the assessment of the potential risks of these substances to humans require experimental systems to measure quantitatively the activity of carcinogens and promoters. Cell culture systems are potentially very useful experimental models for such studies. Short-term, inexpensive cell culture assays for carcinogens are available. These assays have a high predictive ability for the detection of known carcinogens with few false positive results (Barrett et al., 1980). The end point measured, preneoplastic or neoplastic transformation of the test cells, is relevant to the carcinogenic process and is not predicated on a theoretical correlation. These systems can also be used for mechanistic studies on the cellular and molecular basis of neoplastic development. The results with cells and tissues of different species (including human) can be compared and contrasted under similar experimental conditions.


Tracheal Epithelium Chemical Carcinogenesis Respiratory Epithelial Cell Environmental Carcinogen Tracheal Epithelial Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • J. Carl Barrett
    • 1
  • Thomas E. Gray
    • 1
  • Marc J. Mass
    • 1
  • David G. Thomassen
    • 1
  1. 1.Environmental Carcinogenesis Group, Laboratory of Pulmonary Function and ToxicologyNational Institute of Environmental Health SciencesResearch Triangle ParkUSA

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