Sample Collection and Preparation Methods Affecting Mutagenicity and Cytotoxicity of Coal Fly Ash
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Reports by several investigators describing the biological activity of coal fly ash have presented a variety of results which in some cases (Fisher et al., 1979; Clark and Hobbs, 1980; Kubitschek et al., 1980; Mumford and Lewtas, in press) are conflicting. The biological activity of coal fly ash may differ because of one or or more of the following factors: (1) the samples studied were from different sources; (2) the samples were prepared for bioassay differently; (3) the sampling method differed, and, therefore, collected samples were different in chemical or physical properties which affect the biological activity. Several variables involved in coal fly ash studies — source, sample collection and preparation methods, bioassay method — are undoubtedly responsible for the diversity of biological effects observed. The objectives of this study were to examine the sample preparation and collection factors which may affect the observed biological activity caused by coal fly ash and to evaluate the mutagenicity and cytotoxicity of fluidized-bed combustion (FBC) fly ash from experimental and commercial units. The bioassays used in this study were the Ames Salmonella plate incorporation test for mutagenicity and the rabbit alveolar macrophage (RAM) system for cytotoxicity.
KeywordsExtractable Mass Coal Combustion Product Collection Temperature Rabbit Alveolar Macrophage Conventional Combustion
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- Ames, B.W., J.M. McCann, andE. Yamasaki. 1975. Methods for detecting carcinogens and mutagens with the Salmonella/mammalian microsome mutagenicity test. Mutation Res. 31:347–364.Google Scholar
- Claxton, L.D., J. Huisingh, and M. Waters. 1979. The testing of environmental samples for mutagenicity and carcinogenicity using microbial assay systems. In: Symposium Proceedings: Textile Industry Technology, December 1978, Williamsburg, VA. F.A. Ayers, compiler. EPA-600/2–79–104. U.S. Environmental Protection Agency: Research Triangle Park, NC. pp. 231–238.Google Scholar
- Kindya, R.J., R.R. Hall, G. Hunt, W. Piispanen, and P. Fennelly. 1980. Environmental Assessment: Source Test and Evaluation Report -- Exxon Miniplant Pressurized Fluidized-Bed Combustor with Sorbent Regeneration. GCA Corporation: Bedford, MA. pp. 8–25.Google Scholar
- Kubitschek, H.E., D.M. Williams, and F.R. Kirchner. 1980. Biological monitoring of fluidized-bed combustion operations I. Increased mutagenicity during periods of incomplete combustion. In: Short-Term Bioassays in the Analysis of Complex Environmental Mixtures II. M.D. Waters, S.S. Sandhu, J. L. Huisingh, L. Claxton, and S. Nesnow, eds. Plenum Press: New York. pp. 411–420.Google Scholar
- Mumford, J.L., and J. Lewtas. In press. Mutagenicity and cytotoxicity of coal fly ash from fluidized-bed and conventional combustion. J. Toxicol. Environ. Health.Google Scholar
- Young, C.W., P.H. Anderson, R.J. Kindya, R.R. Hall, J.M. Robinson, and P.F. Fennelly. 1981. Environmental Assessment: Source Test and Evaluation Report -- Georgetown University Fluidized-Bed Boiler. GCA-TR-81–76-G. GCA/Technology Division, GCA Corporation: Bedford, MA.Google Scholar