Sample Collection and Preparation Methods Affecting Mutagenicity and Cytotoxicity of Coal Fly Ash

  • Judy L. Mumford
  • Joellen Lewtas
Part of the Environmental Science Research book series (ESRH, volume 27)


Reports by several investigators describing the biological activity of coal fly ash have presented a variety of results which in some cases (Fisher et al., 1979; Clark and Hobbs, 1980; Kubitschek et al., 1980; Mumford and Lewtas, in press) are conflicting. The biological activity of coal fly ash may differ because of one or or more of the following factors: (1) the samples studied were from different sources; (2) the samples were prepared for bioassay differently; (3) the sampling method differed, and, therefore, collected samples were different in chemical or physical properties which affect the biological activity. Several variables involved in coal fly ash studies — source, sample collection and preparation methods, bioassay method — are undoubtedly responsible for the diversity of biological effects observed. The objectives of this study were to examine the sample preparation and collection factors which may affect the observed biological activity caused by coal fly ash and to evaluate the mutagenicity and cytotoxicity of fluidized-bed combustion (FBC) fly ash from experimental and commercial units. The bioassays used in this study were the Ames Salmonella plate incorporation test for mutagenicity and the rabbit alveolar macrophage (RAM) system for cytotoxicity.


Extractable Mass Coal Combustion Product Collection Temperature Rabbit Alveolar Macrophage Conventional Combustion 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Judy L. Mumford
    • 1
  • Joellen Lewtas
    • 1
  1. 1.Genetic Toxicology Division, Health Effects Research LaboratoryU.S. Environmental Protection AgencyResearch Triangle ParkUSA

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