A Purine Auxotroph Deficient in Phosphoribosylpyrophosphate Amidotransferase and Phosphoribosylpyrophosphate Aminotransferase Activities with Normal Activity of Ribose-5-phosphate Aminotransferase

  • E. W. Holmes
  • G. L. King
  • A. Leyva
  • S. C. Singer
Part of the Advances in Experimental Medicine and Biology book series


The synthesis of phosphoribosylamine (PRA) is the first committed reaction unique to purine biosynthesis de novo (Wyngaarden, 1972). Traditionally the catalysis of this reaction has been attributed to the enzyme glutamine phosphoribosylpyrophosphate amidotransferase [E.C.; PP-ribose-P amidotransferase (reaction 1)].


Xanthine Oxidase Mutant Cell Aminotransferase Activity Hand Panel Glutamine Metabolism 
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  1. Chu, E.H.Y., Sun, N. C., and Chang, C. C. 1972. Proc. Nat. Acad. Sci. USA 69: 3459–3463.PubMedCrossRefGoogle Scholar
  2. Feldman, R. I., and Taylor, M. W. 1973. Biochem. Genetics 13: 227–234.CrossRefGoogle Scholar
  3. Fox, I. H. and Kelley, W. N. 1971. J. Biol. Chem. 246: 5739–5748.PubMedGoogle Scholar
  4. Hartman, S. C. 1963. J. Biol. Chem. 238: 3024–3035.PubMedGoogle Scholar
  5. Hartman, S. C. 1973. The enzymes of Glutamine Metabolism, ed. by Prusiner, S. and Stadtman, E. R. (Academic Press, New York) pp. 319–330.Google Scholar
  6. Henderson, J. F. 1963. Biochim. Biophys. Acta 76: 173–180.PubMedCrossRefGoogle Scholar
  7. Holmes, E. W., McDonald, J. A., McCord, J. M., Wyngaarden, J. B. and Kelley, W. N. 1973. J. Biol. Chem. 248: 144–150.PubMedGoogle Scholar
  8. Holmes, E. W., Pehlke, D. M., and Kelley, W. N. 1974. Biochim. Biophys. Acta 364: 209–217.PubMedCrossRefGoogle Scholar
  9. Holmes, E. W., Mason, D. H., Goldstein, L. I., Blount, R. E., Kelley, W. N. 1974. Clin. Chem. 20: 1076–1079.PubMedGoogle Scholar
  10. Kelley, W. N., and Meade, J. C. 1971. J. Biol. Chem. 246: 2953–2958.PubMedGoogle Scholar
  11. King, G. L., and Holmes, E. W, 1975. Anal. Biochem., in press.Google Scholar
  12. Leyva, A. 1974. Thesis for Doctorate in Biochemistry, Duke University Medical CenterGoogle Scholar
  13. Leyva, A. and Kelley, W. N. 1974. Anal. Biochem. 62: 173–179.PubMedCrossRefGoogle Scholar
  14. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. 1951. J. Biol. Chem. 193: 265–275.PubMedGoogle Scholar
  15. Malloy, G. R., Sitz, T. O., and Schmidt, R. R. 1973. J. Biol. Chem. 248: 1970–1975.Google Scholar
  16. Nierlich, D. P., and Magasanik, B. 1965. J. Biol. Chem. 240: 366–374PubMedGoogle Scholar
  17. Ravel, J. M., Eakin, R. E., and Shive, W. 1948. J. Biol. Chem. 172: 67–70.PubMedGoogle Scholar
  18. Reem, G. H. 1968. J. Biol. Chem. 243: 5695–5701.PubMedGoogle Scholar
  19. Reem, G. H. 1972. J. Clin. Invest. 51: 1058–1062.PubMedCrossRefGoogle Scholar
  20. Reem, G. H. 1974. J. Biol. Chem. 249.: 1696–1703.PubMedGoogle Scholar
  21. Reem, G. H., and Friend, C. 1975. Proc. Nat. Acad. Sci. USA 72: 1630–1634.PubMedCrossRefGoogle Scholar
  22. Thomas, C. B., Arnold, W. J., and Kelley, W. N. 1973. J. Biol. Chem. 248: 2529–2535.PubMedGoogle Scholar
  23. Trotta, P. P., Pinkus, L. M., Wellner, V. P., Estis, L., Haschemeyer, R. H., and Meister, A. 1973. The Enzymes of Glutamine Metabolism, ed. by Prusiner, S. and Stadtman, E. R. (Academic Press, New York) pp. 431–482.Google Scholar
  24. Van Der Weyden, M. B., and Kelley, W. N. 1974. J. Biol. Chem. 249: 7282–7289.PubMedGoogle Scholar
  25. Van Der Weyden, M. B., Buckley, R. H., and Kelley, W. N. 1974. Biochem. Biophys. Res. Commun. 57: 590–595.PubMedCrossRefGoogle Scholar
  26. Westby, C. A., and Gots, J. S. 1969. J. Biol. Chem. 244: 2095–2102.PubMedGoogle Scholar
  27. Wyngaarden, J. B. 1972. Current Topics in Cellular Regulation 5: 135–176.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • E. W. Holmes
    • 1
  • G. L. King
    • 1
  • A. Leyva
    • 1
  • S. C. Singer
    • 1
  1. 1.Duke University Medical CenterDurhamUSA

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