Oxypurine and 6-Thiopurine Nucleoside Triphosphate Formation in Human Erythrocytes
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Human erythrocytes are known to form a variety of purine nucleoside triphosphates from the bases and nucleosides, through the action of nucleoside kinases, phosphoribosyltransferases and nucleotide kinases. In addition to ATP and GTP, a very low concentration (6μM) of inosine triphosphate (ITP) was identified in fresh erythrocytes by Vanderheiden (1). More recently, after a 4 hour incubation of stored RBC’s with inosine, Zachara reported that a large amount of ITP was formed (2). A specific ITP pyrophosphohydrolase (3) probably contributes to the low steady state levels of ITP in circulating cells. Inosine has been suggested as a purine source in blood preservation studies (4) and can augment adenine nucleotide pools in studies with ischemic kidneys (5).
KeywordsHigh Performance Liquid Chromatography Nucleoside Triphosphate High Performance Liquid Chromatography Chromatogram High Performance Liquid Chromatography Profile Adenine Nucleotide Pool
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