Stability of the Azaguanine Resistant Phenotype In Vivo
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Deficiency of hypoxanthine guanine phosphoribosyltransferase (HGPRT EC 126.96.36.199) is one of the known causes of drug resistance to 6-mercaptopurine and azaguanine in human leukemias and in experimental tumors in animals. Tumor cells can develop resistance against these agents in the course of treatment; this may reflect either a permanent mutational event or a change in gene expression. Our experiments with virus-induced erythroleukemic cells have shown that gene expression can be altered in vitro (1). Erythroleukemic cells grown in tissue culture could be stimulated by dimethylsulfoxide to differentiate along the erythroid pathway, to synthesize hemoglobin while the activities of enzymes essential to the biosynthesis of purines were repressed in the course of differentiation in vitro.
KeywordsErythroleukemic Cell Cell Specific Marker Mount Sinai School Mouse Cell Line Rodent Cell
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