Stability of the Azaguanine Resistant Phenotype In Vivo

  • Gabrielle H. Reem
  • Charlotte Friend
Part of the Advances in Experimental Medicine and Biology book series


Deficiency of hypoxanthine guanine phosphoribosyltransferase (HGPRT EC is one of the known causes of drug resistance to 6-mercaptopurine and azaguanine in human leukemias and in experimental tumors in animals. Tumor cells can develop resistance against these agents in the course of treatment; this may reflect either a permanent mutational event or a change in gene expression. Our experiments with virus-induced erythroleukemic cells have shown that gene expression can be altered in vitro (1). Erythroleukemic cells grown in tissue culture could be stimulated by dimethylsulfoxide to differentiate along the erythroid pathway, to synthesize hemoglobin while the activities of enzymes essential to the biosynthesis of purines were repressed in the course of differentiation in vitro.


Erythroleukemic Cell Cell Specific Marker Mount Sinai School Mouse Cell Line Rodent Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Reem, G.H. and Friend, C., 1975 Proc. Nat. Acad. Sci. USA 72, 1630–1634.PubMedCrossRefGoogle Scholar
  2. 2.
    Bakay, B., Nyhan, W.L., Croce, C. M. and Koprowski, H., 1975 J. Cell Sci. 17, 567–578.PubMedGoogle Scholar
  3. 3.
    Croce, C.M., Bakay, B., Nyhan, W.L. and Koprowsky, M., 1973, Proc. Nat. Acad. Sci. USA 70, 2590–2594.PubMedCrossRefGoogle Scholar
  4. 4.
    Subak-Sharpe, H., Burk, R.R. and Pitts, J.D., 1969, J. Cell Sci. 4, 353–367.PubMedGoogle Scholar
  5. 5.
    Reem, G. and Friend, C, 1976, J. Cell Sci. 88, 193–196Google Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Gabrielle H. Reem
    • 1
    • 2
  • Charlotte Friend
    • 1
    • 2
  1. 1.Department of PharmacologyNew York University Medical CenterNew YorkUSA
  2. 2.Center for Experimental Cell BiologyMount Sinai School of Medicine of the City University of New YorkN.Y.C.USA

Personalised recommendations