Metabolism of Intravenous Adenine in the Pig
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Although adenine is widely distributed throughout mammalian tissues; either in the form of the energy-rich adenine nucleotides, in combination as the nucleic acids, or the essential enzymes and co-factors such as NAD, FAD; free adenine is almost undetectable in body fluids and tissues under normal circumstances. Since there is no deamination system for adenine at the free base level in mammalian systems (1,6) it can be presumed that all metabolic transformations involving adenine must occur at nucleoside or nucleotide level. An extremely high Km for nucleoside phosphorylase with adenine as substrate makes it extremely unlikely that direct utilisation or formation of adenine normally occurs via this route in mammalian systems (13) (although both the above transformations may be demonstrated in bacterial systems)(6) This being so, the main route for removal of adenine will be to AMP via PRPP and the enzyme APRTase, although endogenous production of free adenine appears unlikely by any of the above routes.
KeywordsUric Acid Xanthine Oxidase Polarise Light Purine Metabolism Adenine Phosphoribosyltransferase
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