Familial Distribution of Increased Erythrocyte PP-Ribose-P Levels
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Studies in vitro and in vivo have suggested the critical role played by the intracellular concentration of PP-ribose-P (PRPP) in the regulation of purine biosynthesis de novo (5,6,14,15). Because abnormalities of PRPP synthesis may contribute to the pathogenesis of some cases of hyperuricemia and gout, we studied the concentration of this compound in a number of gouty patients known to produce excessive quantity of uric acid. Twenty-two male patients, aged 35–72, affected by primary gout with purine overproduction were examined. Control subjects were 11 males, aged 25–45, who were not affected by gout or any other metabolic disease. Fluid intake was not restricted and no subject was taking drugs that might affect urate levels in blood and urine. Serum and urinary uric acid determinations were made by an enzymatic-spectrophotometric method (12). PRPP concentration was determined in erythrocytes and leukocytes according to the method of Micheli et al. (10). HGPRTase activity was assayed in erythrocyte hemolysates by the radiochemical method of Cartier and Hamet (4). The activity of PRPP synthetase was measured in erythrocyte lysates according to the method of Becker et al. (2). PP-ribose-P amidotransferase activity was assayed in leukocyte lysates according to the method of Wyngaarden and Ashton (16).
KeywordsUric Acid Serum Uric Acid Serum Uric Acid Level Glycogen Storage Disease Gouty Arthritis
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- 3.Beutler, E. (1971). In “Red Cell Metabolism”, Grune & Stratton, New York, 64.Google Scholar
- 11.Pompucci, G., Micheli, V., Marcolongo, R. (1976). In press.Google Scholar
- 13.Sperling, O., Boer, P., Persky-Broch, S., Kanarek, E., De Vries, A. (1972). Europ.J.Clin.Biol.Res., 17, 703.Google Scholar
- 14.Sperling, O., Ophir, R., De Vries, A. (1971). Rev.Europ.Et.Clin. Biol., 16, 147.Google Scholar