Incidence of APRT Deficiency
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A partial eficiency of adenine phosphoribosyltransferase (APRT) has been reported in a number of subjects with gout, as well as in asymptomatic individuals (1–4). The association with gout has been attributed to the chance association of two common conditions and no mechanism has been demonstrated to relate APRT deficiency to a disorder of purine metabolism. The incidence of APRT deficiency in 700 normal subjects was assessed to test this hypothesis. A spectrophotometry assay was developed which was ideally suited for large scale surveys. The assay was based on the change of UV-absorbance which resulted on the conversion of Ad to its nucleotide and was dependent upon the simultaneous and quantitative removal of hemoglobin and AMP from the incubation solution. No significant difference was found between the values obtained by this method and the standard radiochemical method (5).
KeywordsNational Health Medical Research Normal Population Medical Research Council Common Condition
- 3.Delbarre, F., Auscher, C., Amor, B. and DeGery, A. (1974). In Sperling, O., DeVries, A. and Wyngaarden, J.B. (Eds) “Purine Metabolism in Man”. Advances in Experimental Medicine and Biology, vol. 41A, Plenum Press, New York, p. 333.Google Scholar