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Phosphoribosylpyrophosphate Synthesis in Human Erythrocytes: Inhibition by Purine Nucleosides

  • Guy Planet
  • Irving H. Fox
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Part of the Advances in Experimental Medicine and Biology book series

Abstract

The intracellular concentration of PP-ribose-P is a balance between its formation and its utilization. Purine nucleosides stimulate PP-ribose-P formation in mammalian cells when inorganic phosphate exceeds 20 mM (1,2). This occurs by the conversion to ribose-5-phosphate of the ribose-1-phosphate liberated from nucleosides by purine nucleoside phosphorylase. Recently Bagnara and Finch (3) showed that nucleosides decreased intracellular PP-ribose-P in Escherichia coli incubated in 1.4 mM inorganic phosphate. The reason for the latter effect has not been clear since there is no substantial direct effect of nucleosides on purified PP-ribose-P synthetase.

Keywords

Human Erythrocyte Adenosine Deaminase Adenylate Kinase Purine Nucleoside Purine Base 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Henderson, J.F. and Khoo, M. 1965. Availability of 5-phosphoribosyl-l-pyrophosphate for ribonucleotide synthesis in Ehrlich ascites tumor cells in vitro. J. Biol. Chem. 240: 2358–2362.PubMedGoogle Scholar
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    Hershko, A., Razin, A. and Mager, J. 1969. Relation of the synthesis of 5-phosphoribosyl-l-pyrophosphate in intact red blood cells and in cell free preparations. Biochim. Biophys. Acta. 184:64–76.PubMedCrossRefGoogle Scholar
  3. 3.
    Bagnara, A.S. and Finch, L.R. 1974. The effects of basis and nucleosides on the intracellular contents of nucleotides and 5-phosphoribosyl-l-pyrophosphate in Escherichia coli. Eur. J. Biochem. 41:421–430.PubMedCrossRefGoogle Scholar
  4. 4.
    Planet, G. and Fox, I.H. 1976. Inhibition of phosphoribosylpyrophosphate synthesis by purine nucleosides in human erythrocytes. J. Biol. Chem. (in press).Google Scholar
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    Fox, I.H. and Kelley, W.N. 1972. Human phosphoribosylpyrophosphate synthetase: kinetic mechanism and product inhibition. J. Biol. Chem. 247:2126–2131.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Guy Planet
    • 1
  • Irving H. Fox
    • 1
  1. 1.Purine Research Laboratory, Rheumatic Disease Unit, Wellesley HospitalUniversity of TorontoTorontoCanada

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