Phosphoribosylpyrophosphate Synthesis in Human Erythrocytes: Inhibition by Purine Nucleosides
- 91 Downloads
The intracellular concentration of PP-ribose-P is a balance between its formation and its utilization. Purine nucleosides stimulate PP-ribose-P formation in mammalian cells when inorganic phosphate exceeds 20 mM (1,2). This occurs by the conversion to ribose-5-phosphate of the ribose-1-phosphate liberated from nucleosides by purine nucleoside phosphorylase. Recently Bagnara and Finch (3) showed that nucleosides decreased intracellular PP-ribose-P in Escherichia coli incubated in 1.4 mM inorganic phosphate. The reason for the latter effect has not been clear since there is no substantial direct effect of nucleosides on purified PP-ribose-P synthetase.
KeywordsHuman Erythrocyte Adenosine Deaminase Adenylate Kinase Purine Nucleoside Purine Base
Unable to display preview. Download preview PDF.
- 4.Planet, G. and Fox, I.H. 1976. Inhibition of phosphoribosylpyrophosphate synthesis by purine nucleosides in human erythrocytes. J. Biol. Chem. (in press).Google Scholar