Phosphoribosylpyrophosphate Synthesis in Human Erythrocytes: Inhibition by Purine Nucleosides

  • Guy Planet
  • Irving H. Fox
Part of the Advances in Experimental Medicine and Biology book series


The intracellular concentration of PP-ribose-P is a balance between its formation and its utilization. Purine nucleosides stimulate PP-ribose-P formation in mammalian cells when inorganic phosphate exceeds 20 mM (1,2). This occurs by the conversion to ribose-5-phosphate of the ribose-1-phosphate liberated from nucleosides by purine nucleoside phosphorylase. Recently Bagnara and Finch (3) showed that nucleosides decreased intracellular PP-ribose-P in Escherichia coli incubated in 1.4 mM inorganic phosphate. The reason for the latter effect has not been clear since there is no substantial direct effect of nucleosides on purified PP-ribose-P synthetase.


Human Erythrocyte Adenosine Deaminase Adenylate Kinase Purine Nucleoside Purine Base 
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  1. 1.
    Henderson, J.F. and Khoo, M. 1965. Availability of 5-phosphoribosyl-l-pyrophosphate for ribonucleotide synthesis in Ehrlich ascites tumor cells in vitro. J. Biol. Chem. 240: 2358–2362.PubMedGoogle Scholar
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    Bagnara, A.S. and Finch, L.R. 1974. The effects of basis and nucleosides on the intracellular contents of nucleotides and 5-phosphoribosyl-l-pyrophosphate in Escherichia coli. Eur. J. Biochem. 41:421–430.PubMedCrossRefGoogle Scholar
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    Planet, G. and Fox, I.H. 1976. Inhibition of phosphoribosylpyrophosphate synthesis by purine nucleosides in human erythrocytes. J. Biol. Chem. (in press).Google Scholar
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    Fox, I.H. and Kelley, W.N. 1972. Human phosphoribosylpyrophosphate synthetase: kinetic mechanism and product inhibition. J. Biol. Chem. 247:2126–2131.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Guy Planet
    • 1
  • Irving H. Fox
    • 1
  1. 1.Purine Research Laboratory, Rheumatic Disease Unit, Wellesley HospitalUniversity of TorontoTorontoCanada

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