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Tissue Microdissection and Processing

  • Anirban Maitra
  • Adi F. Gazdar
Chapter
Part of the Cancer Treatment and Research book series (CTAR, volume 106)

Abstract

The accurate analysis of molecular changes associated with tumors and their precursor lesions requires the precise isolation of the specific cell types from a heterogeneous background of non-neoplastic elements such as normal epithelium, desmoplastic stroma, inflammatory cells and blood vessels(1).In the absence of a prior cell enrichment technique, the results of molecular analysis are undoubtedly confounded by genetic material not derived from cancer cells alone(2).The need for obtaining pure samples of tumor tissue has resulted in the genesis of several methods of cell enrichment including xenograft enrichment,tumor cell lines, cell sorting and microdissection. Xenograft enrichment involves the serial passage of tissues through immunodeficient rodents such asnu/nuor SCID mice to obtain human tumor cell populations whose non-malignant cells are of rodent origin(3, 4, 5).Despite their potential for being an unlimited self-replicating source of high quality genetic material, the ability to propagate xenografts requires considerable expertise, a reasonable animal facility,and time for establishment (between 2 to 6 months)(5).Moreover,there is a possibility that additional genetic changes may be introduced in the tumor cells during serial passage, or a subset of tumor cells with a selective growth advantage may propagate, which may not necessarily be representative of the primary lesion(4,6).Further, the presence of large numbers of stromal cells of rodent origin may complicate molecular analyses. On a similar note, tumor cell lines have been used for a long time to study genetic changes in neoplasia, and are an excellent source of unlimited reagents for this purpose(7,8).Like xenografts however,the establishment of a human tumor cell culture facility requires time,considerable expertise and resources;in addition, the introduction of additional genetic alterations or subset selection are always possibilities(9). Moreover, both cell lines and xenografts are virtually limited to the study of tumor cells only,and preneoplastic lesions have rarely been cultured (10).Cell sorting techniques have also been used at times as a means of cell enrichment, using density gradients, fluoroscence-activated cell sorting or antibody-labeled immunobead selection(11, 12, 13).Cell sorting can be easily applied to tumors amenable to formation of suspensions,such as hematolymphoid malignancies.However,cell sorting techniques are rarely applicable in solid tissue where intercellular adhesion generally prevents the disaggregation of cells,which is a prerequisite for the formation of a cell suspension.

Keywords

Comparative Genomic Hybridization Laser Capture Microdissection Prostatic Intraepithelial Neoplasia Whole Genome Amplification Cancer Chemoprevention 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2001

Authors and Affiliations

  • Anirban Maitra
  • Adi F. Gazdar

There are no affiliations available

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