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Localization and Expression of the Eicosanoid Metabolizing P4501VA Gene Family in Rat Tissues and Hepatic Tumors

  • J. P. Hardwick
  • J. J. Russell
  • S. Kimura
  • F. J. Gonzalez
  • J. K. Reddy
  • C. Peraino
  • E. Huberman
Chapter
  • 56 Downloads
Part of the Developments in Oncology book series (DION, volume 67)

Abstract

The ω-P450 hydroxylase (1VA1-CYP4A) is an eukaryotic gene family(1,2) whose members hydroxylate physiological compounds such as saturated fatty acids, cholesterol, arachidonic acid, leukotrienes and prostaglandins (2,3). Three genes of the rat 1VA gene family have been characterized and shown to be induced by tumor-promoting hypolipidemic drugs (2,3). The physiological function of the ω-oxidized products remains largely undefined. However, the existence of specific P450 isozymes to metabolize these substrates suggests that their induction by tumor promotors may be instrumental in altering the level of autocrine and paracrine mediators of cell growth and differentiation during the development of neoplasia.

Keywords

Western Immunoblot Analysis Cytochrome P450 Isozyme Hepatocellular Tumor Paracrine Mediator Periportal Region 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • J. P. Hardwick
    • 1
    • 2
  • J. J. Russell
    • 1
  • S. Kimura
    • 3
  • F. J. Gonzalez
    • 3
  • J. K. Reddy
    • 4
  • C. Peraino
    • 1
  • E. Huberman
    • 1
  1. 1.Biological and Medical DivisionArgonne National LaboratoriesArgonneUSA
  2. 2.Northeastern Ohio Universities College of MedicineRootstownUSA
  3. 3.Laboratory of Molecular CarcinogenesisNational Cancer Institute, NIHBethesdaUSA
  4. 4.Dept. of PathologyNorthwestern University Medical SchoolChicagoUSA

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