Oxidative Metabolism of Leukocytes and Its Relationship to Bactericidal Activity

  • Lawrence R. DeChatelet
  • Pamela S. Shirley
  • Linda C. McPhail
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 162)


During the course of phagocytosis, normal polymorphonuclear leukocytes undergo remarkable alterations in oxidative metabolism which are insensitive to cyanide or azide. These events, collectively referred to as the respiratory burst, are listed in Table 1. Although neutrophils are equipped with a variety of bactericidal weapons, including non-oxidative processes such as hydrolytic enzymes and cationic proteins, several lines of evidence suggest that the respiratory burst plays a major role in the killing of
Table 1

The Respiratory Burst

I. Increased oxygen consumption

II. Increased glucose oxidation via the hexose monophosphate shunt

III. Generation of hydrogen peroxide

IV. Generation of superoxide anion

V. Generation of chemiluminescence

VI. Reduction of tetrazolium dyes

many bacteria. Bacteria are ingested well under anaerobic conditions but most are not killed efficiently in this situation (1). Further, cells obtained from patients with chronic granulomatous disease fail to elicit a normal respiratory burst and are unable to adequately kill many types of microorganisms (2). Patients with this disease are highly susceptible to severe pyogenic bacterial infections which are frequently life-threatening.


NADPH Oxidase Oxidative Metabolism Respiratory Burst Chronic Granulomatous Disease H202 Production 
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Copyright information

© Plenum Press, New York 1983

Authors and Affiliations

  • Lawrence R. DeChatelet
    • 1
  • Pamela S. Shirley
    • 1
  • Linda C. McPhail
    • 1
  1. 1.The Department of BiochemistryThe Bowman Gray School of MedicineWinston-SalemUSA

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