Identification of Receptors Using Direct Radioligand Binding Techniques

  • Lee E. Limbird


The availability of radioactively labeled hormones and drugs that retain their biological activity has allowed the direct identification of binding sites for these radiolabeled agents in target tissues where they elicit a physiological effect. This chapter will describe methods for the identification of radioligand binding sites and for obtaining data to establish whether or not the observed binding sites represent the physiological “receptor” for a particular hormone, neurotransmitter, or drug.


Radioligand Binding Equilibrium Dialysis Scatchard Plot Competition Binding Positive Cooperativity 
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Copyright information

© Springer Science+Business Media Dordrecht 1986

Authors and Affiliations

  • Lee E. Limbird
    • 1
  1. 1.Vanderbilt UniversityNashvilleUSA

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