Complex Binding Phenomena
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The methods for acquisition and initial analysis of radioligand binding phenomena were summarized in chapter 3. It was demonstrated that equations for linear transformations of binding data were derived assuming that a reversible bimolecular reaction occurred between ligand and receptor and that this interaction obeyed mass action law, namely *D + R ⇌ *DR. Consequently, when data transformations such as the Scatchard plot are nonlinear, when Hill coefficients (nH) do not equal 1.0, or when competition binding curves are not of normal steepness, additional complexities are suggested. Chapter 3 also provided guidelines for evaluating whether or not technical artifacts were responsible for departure of the data from that expected for a simple bimolecular reaction. Once technical artifacts have been excluded, complex binding phenomena suggest the existence of biological complexities which may provide insights into the molecular basis of receptor function.
KeywordsReceptor Subtype Guanine Nucleotide Slope Factor Agonist Binding Receptor Population
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