Multiparameter Light Scattering for Rapid Virus Identification
- 32 Downloads
Multiparameter light scattering (MLS) is a term we have used to describe the simultaneous measurement of multiple elements of the Mueller matrix at specific wavelengths and scattering angles. This 4 Χ 4 matrix describes the polarization sensitive transformation of an incident beam of light into a scattered beam of light by a scattering object such as a virus or suspension of virus particles. The Mueller matrix contains a great deal of information about the internal structure and shape of the virus particle. This information is sufficient in many cases to enable discrimination among a wide variety of different viruses of clinical significance.
KeywordsAzimuthal Angle Rotary Stage Output Window Fast Axis Mueller Matrix
Unable to display preview. Download preview PDF.
- 4.C. F. Bohren and D. R. Huffman. Absorption and scattering of light by small particles. John Wiley and Sons. (1983).Google Scholar
- 5.G. C. Salzman, W. K. Grace, D. M. McGregor and C. T. Gregg, An instrument for virus identification by polarized light scattering: a preliminary report. In: Proceedings of the 4th International Symposium on Rapid Methods and Automation in Microbiology. June 7–10, 1984, Berlin. Springer-Verlag (in press).Google Scholar
- 6.C. T. Gregg, D. M. McGregor, W. K. Grace and G. C. Salzman, Rapid Identification by circular intensity differential scattering. ibid.Google Scholar
- 8.S. Zeitz, A. Belmont and C. Nicolini. Differential scattering of circularly polarized light as a unique probe of polynucleosome superstructure. A simulation by multiple scattering of dipoles. Cell Biophys. 5, 163–187 (1983).Google Scholar