Raman Imaging of Plant Cell Walls

  • Notburga GierlingerEmail author
Part of the Springer Series in Surface Sciences book series (SSSUR, volume 66)


To gain a better understanding of plant cell walls, several microscopic and chemical methods have been used for their analysis in recent years. However, a knowledge gap exists about the location, quantity and structural arrangement of molecules on the micrometer scale within the native cell wall. Advances in confocal Raman microscopy and imaging have tackled this problem in a non-invasive way and provide chemical and structural information in-situ with a high spatial resolution (<0.5 \(\upmu {\mathrm m}\)). Examples of high resolution Raman imaging on wood cells are given, showing that changes in polymer chemistry and orientation can be followed within and between different cell wall layers. In horsetail (Equisetum hyemale) tissue, in addition to the mapping of plant cell wall polymers, the distribution of amorphous silica is investigated. Area scans from a cross section are included as well as a depth profiling within a silica-rich knob on the outer stem wall.



R. Nöske (Potsdam University, Department of Chemistry) is thanked for providing the horsetail sample, B. Clair (Universite Montpellier) for the tropical wood sample and I. Burgert, O. Paris and P. Fratzl (Max Planck Institute of Colloids and Interfaces, Department Biomaterials, Potsdam) for enabling the work. The author acknowledges the APART program of the Austrian Academy of Sciences and the Max Planck Society for funding.


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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  1. 1.Department of NanobiotechnologyUniversität für Bodenkultur WienViennaAustria

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