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High Resolution Optical and Confocal Microscopy

  • Olaf HollricherEmail author
  • Wolfram Ibach
Chapter
  • 1.5k Downloads
Part of the Springer Series in Surface Sciences book series (SSSUR, volume 66)

Abstract

In this chapter, the theory of optical image formation in an optical microscope is described and the difference between conventional and confocal microscopy is explained. The selection of the appropriate pinhole diameter is discussed in detail, as the pinhole diameter is crucial in obtaining the highest depth resolution as well as optimizing collection efficiency, because the Raman signal is typically very weak.

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Copyright information

© Springer International Publishing AG 2018

Authors and Affiliations

  1. 1.WITec GmbHUlmGermany

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