High Resolution Optical and Confocal Microscopy
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In this chapter, the theory of optical image formation in an optical microscope is described and the difference between conventional and confocal microscopy is explained. The selection of the appropriate pinhole diameter is discussed in detail, as the pinhole diameter is crucial in obtaining the highest depth resolution as well as optimizing collection efficiency, because the Raman signal is typically very weak.
- 6.T. Wilson, Confocal Microscopy (Academic Press, 1990), Chap. IV.2. The imaging of simple objects, pp. 25–30Google Scholar
- 7.T. Wilson, Confocal Microscopy (Academic Press, 1990), Chap. IV.2. The imaging of simple objects, pp. 41–45Google Scholar
- 9.C.J.R. Sheppard, M. Gu, Optik 86(3), 104 (1990)Google Scholar