Sortenabhängige Unterschiede der Zusammensetzung der Rhizosphärenbakterienpopulationen bei Raps
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The composition of the bacterial microflora in the rhizosphere of two canola cultivars with either high (‘Rainbow’) or low (‘Monty’) root glucosinolate content was assessed using Biolog ecoplates and denaturing gradient gel electrophoresis (DGGE). After enzymatic breakdown, glucosinolates form toxic substances such as 2-phenylethylisothiocyanate (PEITC) which may affect sensitve microorganisms. Substrate utilization was strongly influenced by soil moisture but not by canola cultivar. Only in ‘Monty’, substrate utilization pattern was affected by PEITC concentration in the rhizosphere soil. The bacterial composition assessed by DGGE was shown to be strongly influenced by PEITC concentration in the rhizosphere soil as well as by soil moisture. The results show that already during growth of canola significant amounts of PEITC are released by the roots which may affect the composition of bacterial rhizosphere microflora.
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