Expression Cloning of cDNA Encoding a Putative Opioid Receptor
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The first demonstration of stereospecific opioid binding to brain membranes was reported 20 years ago (Goldstein et al. 1971), and the decisive demonstration of opioid receptors by ligand binding followed 2 years later (Terenius 1973; Pert and Snyder 1973; Simon et al. 1973; see also Chap. 1, this volume). The first membrane receptor to be cloned was the nicotinic acetylcholine receptor (Cold Spring Harbor Symposium 1983) from the specialized electric organs of electric fish — an extraordinarily rich source compared with ordinary skeletal muscle.
KeywordsOpioid Receptor Opioid Peptide Expression Cloning Electric Fish Chimeric Peptide
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