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The Application of High Throughput Mass Spectrometry to the Analysis of Glycoproteins

  • Sasha Singh
  • Morten Thaysen Andersen
  • Judith Jebanathirajah SteenEmail author
Chapter
  • 799 Downloads

Abstract

Mass spectrometry has significantly contributed to the advancement of glycoprotein research. In combination with conventional glycan purification strategies, high throughput studies become practical, yielding a significant amount of detail regarding the glycoproteome. In this chapter, we provide an overview of commonly employed glycoprotein enrichment strategies which exploit the unique chemical features of glycans for their purification, and introduce the basic concepts of mass spectrometric methods as they apply to glycan identification and characterization. Landmark studies in the enrichment workflows and mass spectrometric analysis of glycans are summarized, as well as the major challenges that face glycoproteomic research.

Keywords

Glycoproteomics Mass spectrometry High throughput Glycopeptides Glycan enrichment techniques 

Abbreviations

bPPCR

Biotinylated phosphine capture reagent

CID

Collision induced dissociation

ECD

Electron capture dissociation

ESI

Electrospray ionization

ETD

Electron transfer dissociation

FAB

Fast atom bombardment

FTICR

Fourier transform ion cyclotron

Gal

Galactose

Gal6

Galectin LEC6

GalNAc

α-N-acetylgalactosamine

Gal-T

β-1,4 galactosyl transferase

Glc

Glucose

GlcNAc

N-acetylglucosamine

HA

Hemagglutinin

HCD

Higher-energy collision dissociation

Hex

Hexose

HILIC

Hydrophilic interaction chromatography

IRMPD

Infrared multiphoton dissociation

IT

Ion trap

LC

Liquid chromatography

MALDI

Matrix assisted laser desorption/ionization

Man

Mannose

MS

Mass spectrometry

MSn

Multi-stage mass spectrometry

NeuGc

N-glycolylneuraminic acid

PAS

Periodate-acid Shiff coupled affinity chromatography

PNGase F

peptide-N-glycosidase F

PTM

Post-translational modification

QIT

Quadrupole ion trap

QqQ

Triple quadrupole

Q-TOF

Quadrupole time of flight

SILAC

Stable isotope labelling with amino acids in cell culture

SLAC

Serial lectin affinity chromatography

TAG

Tagging-via-substrate affinity

TOF

Time of flight

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Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Sasha Singh
    • 1
    • 2
    • 3
  • Morten Thaysen Andersen
    • 4
  • Judith Jebanathirajah Steen
    • 5
    • 1
    • 3
    • 6
    Email author
  1. 1.Proteomics Center at Children’s Hospital BostonBostonUSA
  2. 2.Departments of PathologyHarvard Medical School and Children’s Hospital BostonBostonUSA
  3. 3.F. M. Kirby Neurobiology Center, Children’s Hospital BostonBostonUSA
  4. 4.Department of Biochemistry and Molecular BiologyUniversity of Southern DenmarkOdense MDenmark
  5. 5.F.M. Kirby Center for Neurobiology, Proteomics Center at Children’s Hospital BostonBostonUSA
  6. 6.Department of NeurobiologyHarvard Medical SchoolBostonUSA

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